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Invitrogen™ Oregon Green™ 488 Phalloidin
High-affinity F-actin probe conjugated to green fluorescent Oregon Green 488 dye
Supplier: Invitrogen™ O7466
Description
Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides “death cap” mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while Oregon Green 488 provides green fluorescence with brightness superior to fluorescein. Oregon Green 488 is a fluorinated analog of fluorescein with greater photostability and a lower pKa, making its fluorescence essentially pH insensitive in the physiological pH range. Demonstrating very little nonspecific staining, Oregon Green 488 phalloidin allows high-contrast discrimination of actin staining.
Use in Multiple Applications
Oregon Green 448 phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Oregon Green 448 phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot nanocrystals, and Alexa Fluor conjugates including secondary antibodies.
- Selectively stains F-actin
- Outstanding fluorescence performance
- Excitation/Emission: 496/520nm
- Superior to antibody staining
- Optimal for fixed and permeabilized samples
- pH insensitive under physiological conditions
Cell Analysis, Cell Structure, Cell Tracing & Tracking, Cytoskeleton, Organelle Tracing
Order Info
Shipping Condition: Room Temperature
Specifications
| Store in freezer -5°C to -30°C and protect from light. | |
| Oregon Green™ 488 | |
| Oregon Green™ | |
| Classic Dyes | |
| Actin, Cytoskeleton |
| 496⁄520 | |
| 300 units | |
| Room Temperature | |
| Phalloidin |
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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