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Invitrogen™ OxyBURST™ Green H2DCFDA, SE
Amine-reactive assay reagent
Supplier: Invitrogen™ D2935
Description
OxyBURST™ Green H2DCFDA succinimidyl ester (2',7'-dichlorodihydrofluorescein diacetate, SE) can be used to prepare oxidation-sensitive conjugates of wide variety of biomolecules and particles, including antibodies, antigens, peptides, proteins, dextrans, bacteria, yeast and polystyrene microspheres. Following conjugation to amines, two acetates of OxyBURST™ Green H2DCFDA can be removed by treatment with hydroxylamine at neutral pH to yield dihydrofluorescein conjugate. OxyBURST™ Green H2DCFDA conjugates are nonfluorescent until they are oxidized to corresponding fluorescein derivatives.
- Ex/Em: approx. 492 - 495/517–527nm
- Product is air sensitive (store under dry argon or nitrogen)
- Product may be dissolved in DMSO, DMF, or ethanol for use
- Indicator is cell permeant
- Fluorescence can be monitored using a flow cytometer, fluorometer, microplate reader, or fluorescence microscope, using excitation sources and filters appropriate for fluorescein
Refer to the Molecular Probes™ Handbook for additional product information.
Cell Analysis, Cell Metabolism, Cell Viability, Proliferation and Function, Endocytosis, Exocytosis and Phagocytosis, Nitro-Oxidative Stress, Reactive Oxygen Species
Order Info
Shipping Condition: Room Temperature
Specifications
| 5 mg |
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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