Invitrogen™ SYPRO™ Protein Gel Stains

SYPRO protein gel stains are sensitive, easy-to-use fluorescent stains for total proteins separated by polyacrylamide gel electrophoresis (PAGE).

SYPRO protein gel stains are sensitive, easy-to-use fluorescent stains for total proteins separated by polyacrylamide gel electrophoresis (PAGE). Stained proteins can be viewed with a standard UV or blue-light transilluminator, or imaging equipment containing the appropriate filters or laser. SYPRO protein gel stains offer many advantages over Coomassie Blue and silver staining, including a fast, one-step staining protocol requiring no destaining, a linear dynamic range over three orders of magnitude, and very little protein-to-protein variation in staining.

SYPRO Ruby Protein Gel Stain features include:
• Convenient—ready-to-use formulation
• High sensitivity—detection to 0.25 to 1 ng
• Effective staining for 1D and 2D gels
• Can be multiplexed with other gel stains such as Pro-Q Diamond phosphoprotein gel stain or Pro-Q Emerald 300 glycoprotein gel stain
• Compatible with subsequent analysis of proteins by Edman-based sequencing or mass spectrometry

SYPRO Orange and SYPRO Red protein gel stain features include:
• Sensitive—detection to 4 to 8 ng
• Fast—rapid staining time of 10–60 min
• Best for 1D gels
• SYPRO Orange: Ex: 300, 470 nm, Em: 570 nm
• SYPRO Red: Ex: 300, 550 nm, Em: 630 nm

SYPRO Tangerine Protein Gel Stain features include:
• Sensitive—detection to 4 to 8 ng
• Fast—rapid staining time of 10 to 60 minutes
• Best for 1D gels
• Does not require fixation with acetic acid
• Compatible with subsequent gel staining, western blotting, zymography, electroelution or mass spectrometry

Order Info

Shipping Condition: Room Temperature

Content And Storage

Supplied as a 5000X concentrate in DMSO. Store at room temperature and protect from light.

Description

SYPRO™ Orange Protein Gel Stain (5000X in DMSO), 500 μL

Detection Location

In-Gel Detection

Concentration

5000X in DMSO

Detection Method

Fluorescence

Label or Dye

SYPRO Orange

Product Type

Protein Gel Stain

Product Line

SYPRO™

Quantity

500 μL

Shipping Condition

Room Temperature

Target Molecule

Protein

Excitation/Emission

300, 470/570 nm