Invitrogen™ SYTOX™ Green Nucleic Acid Stain - 5 mM Solution in DMSO

Useful indicator of dead cells within a population

SYTOX Green nucleic acid stain is an excellent green-fluorescent nuclear and chromosome counterstain that is impermeant to live cells.

Simple Viability Determination
SYTOX Green allows quick determination of cell viability when using flow cytometers, fluorescence microscopes, fluorometers, or fluorescence microplate readers. It will not cross intact membranes, but will easily penetrate compromised membranes characteristic of dead cells. Because it exhibits >500-fold fluorescence enhancement upon binding nucleic acids, briefly incubating dead cells with SYTOX Green will result in bright green fluorescence with an emission peak of 523nm when excited by a 488nm argon-ion laser or any other 450–490nm source. The exceptional brightness of the signal produced in dead cells makes SYTOX Green particularly useful for determining viability of not just mammalian cells, but both Gram-positive and Gram-negative bacteria.

Part of the Invitrogen Family of Cell Viability Stains and Assays
SYTOX stains for dead cells are available in a variety of colors, including red, blue, and orange. SYTOX stains have been incorporated into a number of assays for apoptosis, cell viability, and metabolism. 5mM solution in DMSO.

• Impermeant to live cells
• Excitation/Emission: 504/523nm
• Use with 488 Argon-ion laser
• Works with mammalian cells and both Gram-positive and Gram-negative bacteria

Cell Adhesion, Cell Analysis, Cell Cycle, Cell Structure, Cell Tracing and Tracking, Cell Viability and Cytotoxicity, Cell Viability, Proliferation and Function, Cellular Imaging, Flow Cytometry, Flow Cytometry Cell Cycle Assays, Flow Cytometry Staining by Cell Structure, Flow Cytometry Viability and Cytotoxicity Assays, Flow Cytometry of Cellular Processes, Immunofluorescence (IF), Immunofluorescence Counterstaining, Mounting and Fade Prevention, Immunofluorescence Staining and Detection, Microbial Tracking, Nucleus, Nucleoli and Nuclear Envelope, Organelle Tracing

Order Info

Shipping Condition: Room Temperature

Color

Green

Content And Storage

Store in freezer at -5°C to -30°C and protect from light.

Excitation Wavelength Range

504 nm

Dye Type

Cell-Permeant

Format

Tube(s)

For Use With (Equipment)

Flow Cytometer

Quantity

250 μL

Volume (Metric)

250 μL

Detection Method

Fluorescence

Emission

523 nm

Form

Solution

Product Line

SYTOX

Shipping Condition

Room Temperature

Label Type

Fluorescent Dye

Product Type

Nucleic Acid Stain

SubCellular Localization

Nucleic Acids, Nucleus

For Research Use Only. Not for use in diagnostic procedures.