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Gibco™ Mouse (ICR) Inactivated Embryonic Fibroblasts
SDP

Catalog No. A24903
Encompass Procurement Services
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Mouse (ICR) Inactivated Embryonic Fibroblasts

Mouse embryonic fibroblasts (MEFs) are used as a feeder layer when culturing mouse and human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). They are isolated from mouse (strain ICR) embryos. The cells are expanded and inactivated by γ irradiation and cryopreserved. Prior to cryopreservation, the cells are tested for viability (>80% post-thaw viability), verified for growth arrest by plating inactivated cells at low density and monitoring for foci of cell growth after 14 days in culture, and also tested for their ability to support growth of mouse embryonic stem cells (mESCs), human embryonic stem cells (hESCs), and marker expression (Oct 4).

Specifications

Content And Storage

Store in liquid nitrogen.

Age

Fetal

Cell Type

Feeder Cell, Fibroblast Cell

Culture Type

Feeder Dependent

Form

Cryopreserved

Species

Mouse

Donor Source

Pooled

Product Type

Mouse Embryonic Fibroblast

For Research Use Only. Not for use in diagnostic procedures.

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