MP Biomedicals™ 0.25% Trypsin-EDTA (1X)

0.25% Trypsin and 1mM EDTA in modified HBSS w/o Ca++ and Mg++, 100 mL

• Trypsin-EDTA solutions are used to release adherent cells from culture surfaces.
• Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues.
• The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture.
• Ethylenediaminetetracetic acid (EDTA), a chelating agent, is added to trypsin solutions to enhance enzymatic activity by neutralizing calcium and magnesium ions that enhance cellto-cell adhesion and obscure the peptide bonds on which trypsin acts.

Content And Storage

-20°C

Concentration

1:250

Product Type

Cell Culture Dissociation Reagent

Sterility

Sterile

Without Additives

No Calcium, No Magnesium

Formulation

0.25% (w/v) Solution, 0.25% Trypsin

pH

7 to 7.3

Quantity

100 mL

Source

Porcine (for Trypsin)

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use.