MP Biomedicals™ Vitamin D Free Diet, Pellet

Vitamin D Free Diet has been used to study role of Vitamin D in carbohydrate metabolism. Vitamin D deficient diet given to rats at the end of the first week of pregnancy, the suckling pups show evidence of hypocalcemic, hypophosphatemic rickets at age 15-21 day.

Catalog Number	Mfr. No.	Quantity
ICN96007410	MP Biomedicals™ 0296007410	10 kg
ICN96007420	MP Biomedicals™ 0296007420	20 kg
ICN96007450	MP Biomedicals™ 0296007450	50 kg

Description

The diet contains all nutrients (except Vitamin D) required by the rat, and when cholecalciferol is added (5 IU/gm diet), the diet provides growth and reproduction equal to that of commercial stock diets.

• Using whole wheat as the source of carbohydrate the rats experience better growth and development than with mono or disaccharides
• There is some evidence, however, to suggest that Vitamin D depletion may occur more rapidly with sucrose as the carbohydrate source
• The calcium content of the diet can be varied by changing the amount of CaCO3 added
• The phosphorous content can be increased from approximately 0.35% (the wheat flour contains most of the phosphorous and the casein a small and variable amount) by the addition of potassium diphosphate
• When the D deficient diet (which contains 0.4% Ca and 0.4% P) is given to rats (Holtzman Co.) at the end of the first week of pregnancy, the suckling pups show evidence of hypocalcemic, hypophosphatemic rickets at age 15-21 days, as described in A. Boass, W.K. Ramp, and S.U. Toverud: New studies indicate a Glucose diet combined with Calcium restriction or Vitamin D deprivation interferes with the adaptive increase in plasma 1, 25 (OH)2 D3

Specifications

• Product Type: Animal Diet
• Format: Pellet
• Species: Rat

• Components: Vitamin Free Casein 13%, Whole Wheat Flour 75%, Corn Oil 3%, Alphacel, Non-Nutritive Bulk 5%, Calcium Carbonate 0.8%, Calcium Phosphorous Free Salt Mixture ( gran. 1960) 2% Plus MP Vitamin Diet Fortification Mixture except Vitamin D 1.3%
• For Use With (Application): To study role of Vitamin D in Carbohydrate metabolism, Vitamin D deficient diet given to Rats at the end of the first week of pregnancy, the suckling pups show evidence of hypocalcemic, hypophosphatemic Rickets at age 15 to 21 day
• Storage Requirements: 4°C