Invitrogen™ MultiShot™ FlexPlate DH10B T1^R Competent Cells

Includes

Box 1:

• MultiShot™ FlexPlate DH10B™ T1R Chemically Competent E. coli
• One 96-well plate, 20 μl per well

Box 2:

• pUC19 DNA (10 pg/μl): 1 vial, 50 μl
• S.O.C. medium: 2 bottles, 10 ml each

MultiShot FlexPlate DH10B T1^R chemically competent E. coli cells are cloning competent cells pre-aliquoted in a 96-well PCR plate to increase transformation productivity.

MultiShot FlexPlate DH10B T1^R chemically competent E. coli cells are cloning competent cells pre-aliquoted in a 96-well PCR plate to increase transformation productivity. The FlexPlate format can be conveniently divided into 8-well segments for flexibility in transformation throughput.

All of our T1^R strains have the tonA (fhuA) deletion in their genotype that confers resistance to T1 and T5 phage. T1 bacteriophage spread rapidly and lyse E. coli hosts, which are commonly used for cloning and library construction. A resistant genotype offers added security to protect valuable clones and libraries. This is especially important for genome and sequencing centers where T1 phage infection would be catastrophic.

Diverse DH10B T1^R cloning capabilities
MultiShot FlexPlate DH10B T1R competent cells may be used in a variety of cloning applications as they are resistant to the effects of ligase and ligation buffers and provide transformation efficiencies of >1 x 10⁸ cfu/μg with control plasmid DNA. The cells are ideal for cloning large plasmids and BACs and are suitable for cloning DNA that contains methylcytosine and methyladenine (i.e., genomic DNA).

MultiShot FlexPlate DH10B T1R Competent Cell features
• mcrA, mcrBC, mrr, and hsdRMS elimination of restriction systems to allow construction of more representative genomic constructs
• tonA (fhuA) genotype to confer resistance to T1 and T5 phage
• endA1 for increased plasmid yield and quantity
• recA1 for reduced occurrence of non-specific recombination in cloned DNA
• lacZΔM15 for blue/white color screening of recombinant clones

Increase transformation productivity with MultiShot FlexWell format
The FlexPlate is a 96-well PCR plate that can be used in automated liquid handling systems or divided into 8-well segments for flexibility in transformation throughput. Addition of DNA to the MultiShot FlexPlate Competent Cells can be quickly performed with a multichannel pipet or a liquid handling system. After addition of DNA, the cells can be efficiently transformed by heat-shock using a water bath, heat block, or thermal cycler.

Key benefits of the MultiShot FlexPlate format include:
• Increase throughput—compatible with automated liquid handling platforms
• Flexible 96-well plate may be divided into 8-wellsstrips
• Competent cells come pre-aliquoted in 96-well plates and ready for transformation

Genotype
F^- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 lacX74 recA1 endA1 araD139 Δ(ara-leu)7697 galU galK rpsL nupG λ- tonA

Find the strain and format that you need
• We offer many E. coli strains and formats of chemically competent and electrocompetent cells to meet your specific transformation needs.
• Choose from our MultiShot formatted comp cells for high-throughput options.

Product Type

Chemically Competent Cells

Contains F' Episome

No

Cloning Methylated DNA

Yes

Content And Storage

• MultiShot FlexPlate DH10B T1^R Competent Cells (1 x 96-well plate at 20 μL/well)
Store at –80°C.

• pUC19 DNA (50 μL at 10 pg/μL)
Store at –20°C.

• S.O.C. Medium (2 x 10 mL)
Store at 4°C or room temperature.

Antibiotic Resistance Bacterial

No

Blue/White Screening

Yes (lacZΔM15)

High-throughput Compatibility

High

Plasmid

May be used for plasmids >100 kb

Preparing Unmethylated DNA

No

Reduces Recombination

Yes (recA1)

Shipping Condition

Dry Ice

T1 Phage - Resistant (tonA)

Yes

Species

E. coli (K12)

Format

96-well Plate

Product Line

Multishot™

Quantity

1 x 96-well Plate

For Research Use Only. Not for use in diagnostic procedures.