Cytiva NHS Mag Sepharose™

NHS Mag Sepharose magnetic beads for convenient enrichment of selected proteins using pull-down sample preparation techniques

NHS Mag Sepharose are magnetic beads designed for pull-down techniques enabling rapid capture and enrichment of selected proteins based on affinity. An antibody or other protein with desired affinity is covalently coupled to the medium via primary amines for the purpose of capture of target proteins of interest.

• Visible and dense Sepharose based magnetic beads with N-hydroxysuccinimide (NHS) ligand, easy to spot and collect bound target protein
• Non-adherent beads eliminate smearing effects and aggregate formation. Can be used without detergents
• Simple capture of target protein in small or large sample volumes (low microliter to high milliliter scale)
• Optimized capacity for enrichment/pull-down of target proteins - only low amounts of bound capture molecule
• Flexible protocols with elution conditions optimized for downstream applications such as electrophoresis and MS

Matrix: Paramagnetic, spherical, highly cross-linked agarose particles
Ligand: N-hydroxysuccinimide
Ligand density: 8-14μmol/mL gel
Particle size: 37-100μm
Working temperature: Room temperature
Storage solution: 20% 2-propanol
Storage temperature: 4°C to 8°C

Bead Type

NHS Mag Sepharose

Binding Capacity

8 to 14μM/mL

Ligand Concentration

8 to 14μM/mL

Matrix

Paramagnetic spherical, highly cross linked agarose particles

Particle Size

37 to 100 μm

Quantity

4 x 500 μL

Content And Storage

4°C to 8°C

For Use With (Application)

Designed for covalent coupling of any Protein or biomolecule containing a free Amine group and is used for various types of pull down experiments, for biomarker research

Ligand

N-hydroxysuccinimide