Normal Human Serum (C4 free), Invitrogen™

Rabbit Control

Titer results: Gel double diffusion titer : 1:>32. Gel double diffusion titer is defined as the dilution of antiserum sufficient to form a precipitate arc with 5 μg antigen and 5 μL per well.

Normal Sera are non-immune serum samples from goat, human, mouse, rabbit and other species that contain the usual complement of serum proteins, including the various classes of immunoglobulins that exist in healthy animals of the particular species. Normal serum provides a sufficient quantity of endogenous proteins to saturate and block nonspecific binding sites on fixed samples or assay substrates. When used as an antibody diluent, non-immune serum also supplies a native chemical environment for antibodies to function properly without adding foreign protein components. Normal serum is frequently used for blocking or saturating generalized binding interactions for immunodetection methods, especially those involving tissue samples such as immunohistochemistry (IHC). Normal sera are also useful as controls for testing general and specific antibody purification methods.

Content And Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Form

Liquid

Product Type

Normal Human Serum (C4 free)

Quantity

1.5 mL

Concentration

Conc. Not Determined

Formulation

whole serum with 0.1% sodium azide

For Use With (Application)

Immunodiffusion, Western Blot, ELISA, Immunoelectrophoresis

Isotype

Ig

Regulatory Status

RUO