MilliporeSigma™ Novagen™ Overnight Express™ Autoinduction NMR Medium - ¹⁵N ¹³C

Used to optimize cell growth and protein expression before preparing isotopically labeled proteins for NMR spectroscopy. MilliporeSigma™; Novagen™ Overnight Express™ Autoinduction NMR Optimization Medium is based on the differential metabolism of carbon sources to promote growth to high cell density.

• Automatically induce protein expression under control of lac promoters, eliminating the need to monitor cell densities or add inducer (IPTG)
• Includes four concentrated sterile solutions:
• OnEx Dual-NMR Solution 1 is a blend of carbon sources optimized for tightly regulated uninduced growth to high density, followed by autoinduction
• OnEx NMR Solution 2 is a buffer and nitrogen blend that mediates metabolic acid production and provides additional nitrogen for increased protein synthesis
• OnEx Solution 3 provides magnesium for maximal cell density
• OnEx Solution 4 provides trace metals to minimize growth limitations associated with mineral deficiencies and satisfy the requirements of metal-containing target proteins, even at high expression levels

Quantity

1 L Kit

Content And Storage

Multiple storage requirements. Ok to freeze.

Product Type

Autoinduction NMR Medium - ¹⁵N ¹³C