Invitrogen™ Novex™ 10 to 20%, Tricine, 1.0 mm, Mini Protein Gels

Includes

Catalog numbers for gels refer to a single box. One box contains 10 gels.

Invitrogen Novex Tricine Gels provide separation of low molecular weight proteins and peptides. In this system tricine replaces glycine in the running buffer, resulting in more efficient stacking and destacking for low molecular weight proteins and higher resolution of smaller peptides.

Invitrogen Novex Tricine Gels provide separation of low molecular weight proteins and peptides. The Tricine system is a modification of the tris-glycine discontinuous buffer system developed by Schaegger and von Jagow (Schaegger and von Jagow, 1987) specifically for resolving peptides and low molecular weight proteins. In this system the tricine replaces the glycine in the running buffer, resulting in more efficient stacking and destacking for low molecular weight proteins and higher resolution of smaller peptides.

Features of Novex Tricine protein gels:
• Increased resolution of proteins with molecular weights as low as 2 kDa
• Improved compatibility with direct sequencing of proteins after transferring to PVDF
• Minimized protein modification due to the lower pH of the tricine buffering system

Formulation
Invitrogen Tricine gels are made with high-purity, strictly quality-controlled reagents: Tris base, HCl, acrylamide, bisacrylamide, TEMED, APS, and highly purified water. Our Tricine gels have a 4% stacking gel and do not contain SDS. The Tricine system requires SDS in sample and running buffers for best results.

Choose the right Tricine gel for your protein separation
Invitrogen Tricine gels come in three polyacrylamide concentrations of 10%, 16%, and a gradient of 10–20%. Select from our many well formats, including 10-, 12-, and 15-well. Tricine gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, we recommend Tricine SDS Sample Buffer (LC1676) and optimal separation use Tricine SDS Running Buffer (LC1675).

For transfer of proteins to a membrane, we recommend using the Novex Tris-Glycine Transfer Buffer (LC3675) if performing a traditional wet transfer using the XCell II Blot Module (EI9051) or the Mini Blot Module (B1000). Rapid semi-dry transfer can be performed using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device (IB21001).

Order Info

Shipping Condition: Wet Ice

For Use With (Equipment)

Mini Gel Tank, XCell SureLock Mini-Cell

Gel Percentage

10 to 20%

Gel Size

Mini

Gel Thickness

1.0 mm

Gel Type

Tricine

Recommended Applications

Denaturing

Sample Loading Volume

Up to 25 μL

Separation Range

2.5 to 250 kDa

Separation Type

Denaturing

Wells

10-well

Mode of Separation

Molecular Weight

Product Line

Novex™

Quantity

10 Gels/Box

Shipping Condition

Wet Ice

Storage Requirements

Store at 2°C to 8°C. Do not freeze.

Length (Metric)

8 cm

Width (Metric)

8 cm