Invitrogen™ Novex™ Tris-Glycine Plus Midi Protein Gels, 4 to 20%, 1.0 mm

Novex Tris-Glycine Plus Midi Protein gels are improved polyacrylamide gels based on traditional Laemmli protein electrophoresis technology, allowing the use of Laemmli sample and running buffers.

Novex Tris-Glycine Plus Midi Protein gels are improved polyacrylamide gels based on traditional Laemmli protein electrophoresis technology, allowing the use of Laemmli sample and running buffers. The gels offer reproducible separation of a wide range of proteins into well-resolved bands. Novex Tris-Glycine Plus Midi gels are a 1.0-mm thick, wider format (8 cm x 13 cm) gel for higher throughput electrophoresis of protein samples.

Features of Novex Tris-Glycine Plus gels include:
Improved shelf life—store gels for up to 12 months at 4°C
Flexibility—use for native and denaturing protein assays
Fast run conditions—quickly separate your proteins using constant voltage in less than 60 minutes

Choose the right gel format for your experiments
Novex Tris-Glycine Plus Midi gels come in fixed concentrations of 10% and 12%, as well as gradient concentrations of 4–12%, 4–20%, and 8–16%. Select from our many well formats, including 12+2-, 20-, and 26-well.

Run your proteins in native or denatured form
Novex Tris-Glycine Plus gels do not contain SDS and so can be used to separate proteins in native or denatured form. For denatured proteins, we recommend using Novex Tris-Glycine SDS Sample Buffer (LC2676) and Novex Tris-Glycine SDS Running Buffer (LC26754). For native proteins, we recommend using Novex Tris-Glycine Native Sample Buffer (LC2673) and Novex Tris-Glycine Native Running Buffer (LC2672). The gels can be run using our XCell4 SureLock Midi-Cell (WR0100) or conveniently with the Bio-Rad Criterion Cell using our adapters (WA0999).

For transfer of proteins to a membrane, we recommend using the Novex Tris-Glycine Transfer Buffer (LC3675) when performing traditional wet transfer. Rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device (IB21001).

Recommended Storage

Store in refrigerator (2-8°C). Do not freeze.

Adapters

No Adapters

For Use With (Equipment)

XCell4 SureLock Midi-Cell, SureLock Tandem Midi Gel Tank

Gel Percentage

4 to 20%

Gel Size

Midi

Gel Thickness

1.0 mm

Gel Type

Tris-Glycine Plus

Sample Loading Volume

Up to 45 μL + 15 μL

Separation Range

20 to 200 kDa

Separation Type

Denaturing, Native

Wells

12 + 2-well

Mode of Separation

Molecular Weight

Product Line

Novex™

Quantity

10 Gels/Box

Shipping Condition

Wet Ice

Storage Requirements

Store at 2°C to 8°C. Do not freeze.

Length (Metric)

8 cm

Width (Metric)

13 cm