Invitrogen™ IL-1 beta Human ELISA Kit

Quantifies natural and recombinant human IL-1β in serum, buffered solution, or cell culture medium

The Human Interleukin-1beta (Hu IL-1beta) ELISA quantitates Hu IL-1β in human serum, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-1β. Principle of the method The Human IL-1β solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.

Accession Number

P01584

Assay Range

3.9-250 pg/mL

Assay Sensitivity

1 pg/mL

Conjugate

Biotin

Product Type

ELISA Kit

Sample Type

Serum, Supernatant

Target Species

Human

For Use With (Application)

Colorimetric

For Use With (Equipment)

Colorimetric Microplate Reader

Gene ID (Entrez)

3553

Gene Symbol

IL-1,IL1-BETA,IL1F2

Interassay CV

6.6%

Intraassay CV

4.4%

Kit Contents

Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers

Label or Dye

HRP

Precision

1pg/mL

Quantity

96 Tests

Regulatory Status

RUO

Sample Volume

Serum, 50 μL; Supernatant, 50 μL

Storage Requirements

2°C to 8°C

Target

Human

Test Time

1 hr. 20 min.

Total Assay Time

2 hr. 55 min.