Invitrogen™ IL-6 Mouse ELISA Kit

Includes

Mouse IL-6 Antibody Coated 96-Well Plate, Mouse IL-6 Standard, Standard Diluent Buffer, Mouse IL-6 Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer Concentrate (25X), Stabilized Chromogen, TMB, Stop Solution, Plate Covers, Detailed protocol with validation tests

To detect and quantify the level of mouse IL-6 in serum, plasma, and supernatant using 96-well plates and a microplate reader

The Mouse Interleukin (IL-6)) ELISA quantitates Ms IL-6 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IL-6. Principle of the method The Mouse IL-6 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Interleukin 6 (IL-6) is a multifunctional 26 kD protein originally discovered in the medium of RNA-stimulated fibroblastoid cells. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be important as IL-1 and TNF-a in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. IL-6 acts upon a variety of cells including myeloid progenitor cells, T cells, B cells, and hepatocytes. In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. IL-6 plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. IL-6 is a very useful culture supplement for the generation of a high number of antibody-producing hybridomas. Primarily produced at sites of acute and chronic inflammation, IL-6 is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of IL-6 is implicated in a wide variety of inflammation-associated disease states including diabetes mellitus and systemic juvenile rheumatoid arthritis.

Order Info

Shipping Condition: Wet ice

Accession Number

P08505

Assay Range

7.8-500 pg/mL

Assay Sensitivity

<3 pg/mL

Conjugate

Biotin

Product Type

ELISA Kit

Sample Type

Plasma, Serum, Supernatant

Target Species

Mouse

For Use With (Application)

Colorimetric

For Use With (Equipment)

Colorimetric Microplate Reader

Gene ID (Entrez)

16193

Gene Symbol

IL-6

Interassay CV

6.7%

Intraassay CV

5.8%

Kit Contents

Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers

Label or Dye

HRP

Precision

<3pg/mL

Quantity

96 Tests

Regulatory Status

RUO

Sample Volume

Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL

Storage Requirements

2°C to 8°C

Target

Mouse

Test Time

1 hr. 20 min.

Total Assay Time

3 hr. 30 min.

For Research Use Only. Not for use in diagnostic procedures.