Invitrogen™ ELISA Stop Solution

A ready-to-use solution intended for use as a stop solution in ELISA applications that use horseradish peroxidase and TMB substrate solution.

• A ready-to-use solution intended for use as a stop solution in ELISA applications that use horseradish peroxidase and TMB substrate solution
• As TMB substrate solution reacts with horseradish peroxidase, it produces a pronounced blue color
• At the end of TMB incubation, a volume of ELISA Stop Solution equal to the volume of TMB substrate solution is added to the wells to terminate the horseradish peroxidase reaction
• Addition of Stop Solution results in the rapid formation of a yellow end product
• The optical density of this yellow end product is measured at 450 nm
• The yellow end product is stable for sixty minutes after addition of Stop Solution, optical density should be measured within this time period
• Store at 2°C to 8°C

Order Info

Shipping Condition: Room Temperature

Product Type

ELISA Stop Solution

Quantity

25 mL

Form

Liquid

Substrate Type

HRP, TMB

Content And Storage

Store at 2°C to 8°C

Shipping Condition

Wet Ice

For Research Use Only. Not for use in diagnostic procedures.