Invitrogen™ NuPAGE™ Bis-Tris Mini Protein Gels, 12%, 1.0 mm

Includes

One box containing 10 gels

NuPAGE™ Novex™ 12% Bis-Tris Gels are pre-cast polyacrylamide gels designed to give optimal separation of small- to medium-sized proteins under denaturing conditions.

NuPAGE™ Novex™ 12% Bis-Tris Gels are pre-cast polyacrylamide gels designed to give optimal separation of your small- to medium-sized proteins under denaturing conditions. Unlike traditional Tris-Glycine gels, NuPAGE™ Novex™ Bis-Tris gels have a neutral pH environment that minimizes protein modifications. Use NuPAGE™ Novex™ Bis-Tris gels for preparing proteins for sequencing, mass spectrometry, and any other techniques where protein integrity is important.

Features of NuPAGE Bis-Tris gels:
• Better protein integrity—optimized sample preparation process preserves your proteins
• Wide ranges of molecular weight separation—select the right gel and running buffer to get the optimal separation of your proteins
• Faster run times—get separation of your proteins in as little as 35 minutes
• Longer shelf life—NuPAGE Bis-Tris gels can be stored for at least 12 months at room temperature

Choose the right NuPAGE Bis-Tris gel for your protein separation
Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. NuPAGE Bis-Tris protein gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and a 4–12% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Bis-Tris gels also come in multiple well formats.

NuPAGE Bis-Tris gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, use the NuPAGE LDS Sample Buffer (NP0007) and NuPAGE Sample Reducing Agent (NP0004). Use NuPAGE Antioxidant (NP0005) in the running buffer to maintain the reduced state of the proteins during the run and to allow maximum band sharpness. The gels can be run using NuPAGE MES SDS Running Buffer (NP0002) to better resolve small proteins or NuPAGE MOPS SDS Running Buffer (NP000102) to resolve medium- to large-size proteins.

For transfer of proteins to a membrane, we recommend using NuPAGE Transfer Buffer (NP00061) for traditional wet transfer using the Mini Blot Module (B1000) or XCell II Blot Module (EI9051). Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device (IB210010).

Order Info

Shipping Condition: Room Temperature

For Use With (Equipment)

Mini Gel Tank, XCell SureLock Mini-Cell

Gel Percentage

12%

Gel Size

Mini

Gel Thickness

1.0 mm

Gel Type

Bis-Tris

Recommended Applications

Denaturing

Sample Loading Volume

Up to 25 μL

Separation Range

3.5 to 80 kDa

Separation Type

Denaturing

Wells

10-well

Mode of Separation

Molecular Weight

Product Line

NuPAGE™

Quantity

10 Gels/Box

Shipping Condition

Room Temperature

Storage Requirements

Store at 4–25°C. Do not freeze.

Length (Metric)

8 cm

Width (Metric)

8 cm