Thermo Scientific™ PageRuler™ Plus Prestained Protein Ladder, 10 to 250 kDa

Thermo Scientific™ PageRuler Plus Prestained Protein Ladder is a mixture of nine (9) blue-, orange- and green-stained proteins (10 to 250 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

Thermo Scientific PageRuler Plus Prestained Protein Ladder is a mixture of nine blue-, orange-, and green-stained proteins (10 to 250 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting. The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

Applications

• Monitoring protein migration during SDS-polyacrylamide gel electrophoresis
• Monitoring protein transfer onto membranes after western blotting
• Sizing of proteins on SDS-polyacrylamide gels and western blots

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Content And Storage

Contents: two vials of 250 μL each

Storage buffer: 62.5 mM Tris-H₃PO₄ (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN₃ and 33% glycerol

Storage: Upon receipt store at -20°C

Detection Method

Colorimetric, NIR Fluorescence (700 nm), RGB Fluorescence (555 nm)

Number of Markers

9

Product Type

Protein Ladder

Ready to Load

Yes

Size Range

10 to 250 kDa

Stain Type

3 colors: Blue, Orange, Green

Gel Compatibility

Bolt™ Bis-Tris Plus Gels, Novex™ Tricine Gels, Novex™ Tris-Glycine Gels, NuPAGE™ Bis-Tris Gels, NuPAGE™ Tris-Acetate Gels, SDS-PAGE Gels

Molecular Weight (g/mol)

250, 130, 100, 70, 55, 35, 25, 15, 10 kDa

Product Line

PageRuler™

Quantity

2 x 250 μL

Shipping Condition

Approved for shipment on Wet or Dry Ice

System Type

Western Blotting, SDS-PAGE

For Research Use Only. Not for use in diagnostic procedures.