Thermo Scientific™ PageRuler™ Prestained NIR Protein Ladder

A mixture of 10 proteins (11 to 250KDa) that are blue-stained and fluor-labeled for near-IR fluorescent visualization and protein sizing following electrophoresis.

The Thermo Scientific™ PageRuler Prestained NIR Protein Ladder is a mixture of 10 proteins (11 to 250KDa) that are blue-stained and fluor-labeled for near-IR fluorescent visualization and protein sizing following electrophoresis.

The protein MW markers in this ladder resolve into sharp bands when analyzed by SDS-PAGE and are labeled with a fluorescent dye for visualization with instruments equipped for detection of near-infrared (NIR) fluorescence. The bands are also directly visible because the proteins are prestained blue. The 55kDa band is of greater intensity and serves as a reference band. The protein ladder is conveniently packaged and ready to use: no heating, dilution or addition of a reducing agent is required before use.

Highlights:

• Size range 10 proteins spanning 11 to 250kDa

• Fluorescent detect using 670nm red laser or 700nm channels with fluorescence imagers

• Ready-to-use supplied in a loading buffer for direct loading on gels

• Sharp bands excellent sizing for near-infrared visualization of protein gels and blots

• Reference band 55kDa band has greater intensity for easy orientation

• Quality tested each lot evaluated by SDS-PAGE and Western blotting

Includes:

Proteins in 62.5mM Tris-H₃PO₄ (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 10mM DTT, 1mM NaN₃ and 33% glycerol

Requires:

Fluorescent imager capable of near-infrared fluorescence detection, such as certain Typhoon™ Imagers and the LI-COR Odyssey™ Infrared Imaging System

Recommended for:

Protein sizing on SDS-polyacrylamide gels and Western blots for visualization with near-infrared (NIR) fluorescence imagers

Content And Storage

Contents: two vials of 250 μL each

Storage buffer: 62.5 mM Tris-H₃PO₄ (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN₃ and 33% glycerol

Storage: Upon receipt store at -20°C

Detection Method

Colorimetric, NIR Fluorescence (700 nm)

Number of Markers

10

Product Type

Protein Ladder

Ready to Load

Yes

Size Range

11 to 250 kDa

Stain Type

1 color: Blue

Gel Compatibility

Bolt™ Bis-Tris Plus Gels, Novex™ Tricine Gels, Novex™ Tris-Glycine Gels, NuPAGE™ Bis-Tris Gels, NuPAGE™ Tris-Acetate Gels, SDS-PAGE Gels

Molecular Weight (g/mol)

250, 130, 95, 70, 55, 43, 34, 26, 15, 11 kDa

Product Line

PageRuler™

Quantity

2 x 250 mL

Shipping Condition

Approved for shipment on Wet or Dry Ice

System Type

Western Blotting, SDS-PAGE

For Research Use Only. Not for use in diagnostic procedures.