Thermo Scientific™ PageRuler™ Unstained Broad Range Protein Ladder

A mixture of 11 proteins (5 to 250kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

Thermo Scientific™ PageRuler Unstained Broad Range Protein Ladder is a mixture of 11 proteins (5 to 250kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

The protein MW markers in this ladder resolve into clearly identifiable sharp bands ranging in size from 5kDa to 250kDa when analyzed by SDS-PAGE and stained with coomassie dye or silver. The 100, 50 and 20kDa protein bands are of greater intensity and serve as reference bands. Proteins can be detected on Western blots by staining with Ponceau S, coomassie blue or other protein stains. In addition, the ladder proteins (except for the 5kDa peptide) contain an integral Strep-tag™ II Sequence and may be detected on Western blots using Strep-Tactin™ Conjugates. The PageRuler Unstained Broad Range Protein Ladder is supplied ready-to-use: no heating, dilution or addition of a reducing agent is required before use.

Highlights:

• Size range – eleven proteins spanning 5 to 250kDa

• Ready-to-use – supplied in a loading buffer for direct loading on gels; no need to boil

• Sharp bands – excellent accuracy for detection in with coomassie or silver stains

• Reference bands – the 100, 50 and 20kDa bands are more intense for easy orientation

• Tagged – each protein in the ladder contains an integral Strep-tag™ II Sequence

• Quality tested – each lot evaluated by SDS-PAGE and Western blotting

Includes:

Proteins in 62.5mM Tris-H₃PO₄ (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 100mM DTT, 1mM NaN₃, 0.01% bromophenol blue and 33% glycerol.

Recommended for:

Accurate protein sizing on SDS-polyacrylamide gels and Western blots

Content And Storage

Contents: two vials of 250 μL each

Storage buffer: 62.5 mM Tris-H₃PO₄ (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN₃, 0.01% bromophenol blue and 33% glycerol

Storage: Upon receipt store at -20°C

Number of Markers

11

Product Type

Protein Ladder

Ready to Load

Yes

Size Range

5 to 250 kDa

Stain Type

Unstained

Gel Compatibility

Bolt™ Bis-Tris Plus Gels, Novex™ Tricine Gels, Novex™ Tris-Glycine Gels, NuPAGE™ Bis-Tris Gels, NuPAGE™ Tris-Acetate Gels, SDS-PAGE Gels

Molecular Weight (g/mol)

250, 150, 100, 70, 50, 40, 30, 20, 15, 10, 5 kDa

Product Line

PageRuler™

Quantity

2 x 250 μL

Shipping Condition

Approved for shipment on Wet or Dry Ice

System Type

Western Blotting, SDS-PAGE

For Research Use Only. Not for use in diagnostic procedures.