Thermo Scientific™ PageRuler™ Unstained Protein Ladder

A mixture of seven native proteins (14.4 to 116kDa) for use as size standards in protein electrophoresis (SDS-PAGE).

Thermo Scientific PageRuler Unstained Protein Ladder is a mixture of 14 recombinant, highly purified, unstained proteins (10 to 200 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting. The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

Features of the PageRuler Unstained Protein Ladder include:

• Reference bands—50 kDa band is stronger than the others for easy orientation
• Tagged—the ladder proteins contains an integral Strep-tag™ II sequence and can be detected on western blots using Strep-Tactin™ conjugates or an antibody against Strep-tag™ II Sequence

Applications

• Accurate protein sizing on SDS-polyacrylamide gels and western blots

Content And Storage

Contents: two vials of 250 μL each, proteins at 0.02 to 0.05 mg/mL of each

Storage buffer: 62.5 mM Tris-H₃PO₄ (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN₃, 0.01% bromophenol blue and 33% glycerol

Storage: Upon receipt store at -20°C

Number of Markers

14

Product Type

Protein Ladder

Ready to Load

Yes

Size Range

10 to 200 kDa

Stain Type

Unstained

Gel Compatibility

Bolt™ Bis-Tris Plus Gels, Novex™ Tricine Gels, Novex™ Tris-Glycine Gels, NuPAGE™ Bis-Tris Gels, NuPAGE™ Tris-Acetate Gels, SDS-PAGE Gels

Molecular Weight (g/mol)

200, 150, 120, 100, 85, 70, 60, 50, 40, 30, 25, 20, 15, 10 kDa

Product Line

PageRuler™

Quantity

2 x 250 mL

Shipping Condition

Approved for shipment on Wet or Dry Ice

System Type

Western Blotting, SDS-PAGE

For Research Use Only. Not for use in diagnostic procedures.