Invitrogen™ PeproGMP® Human EGF Recombinant Protein, PeproTech®

Recombinant Protein

EGF (Epidermal growth factor) exerts its actions by binding to the EGF Receptor, a 170 kDa protein kinase. Activation of EGFR initiates diverse cellular pathways in response to toxic environmental stimuli, or to EGF binding to the receptor, the EGFR forms homo- or heterodimers with other family members. Each dimeric receptor complex initiates a distinct signaling pathway by recruiting different Src homology 2 (SH2) containing effector proteins. EGF is far and wide expressed in kidney, cerebrum, prostrate and salivary glands. EGF acts as a potent mitogenic factor and the phosphorylated receptor recruits adapter proteins like GRB2 that activates complex downstream signaling cascades. EGF activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STAT modules. Research studies suggest the protein may also play important role in activating the NF-kappa-B signaling cascade. Defects in the EGGF gene are the cause of hypomagnesemia type 4 and dysregulation has been associated with the growth and progression of certain cancers.

Accession Number

P01133

For Use With (Application)

Functional Assay

Format

Lyophilized

Formulation

Protein with no preservative

Gene ID (Entrez)

1950

Molecular Weight (g/mol)

6.2 kDa

Name

EGF

Purification Method

HPLC, SDS-PAGE

Quantity

1 mg

Storage Requirements

-20°C or to +80°C if preferred

Regulatory Status

RUO

Endotoxin Concentration

<0.1 EU/ μg

Gene Alias

AI790464; beta-urogastrone; EGF; Epidermal growth factor; epidermal growth factor (beta-urogastrone); epidermal growth factor precursor; H-EGF; HOMG4; Pro-epidermal growth factor; Pro-epidermal growth factor precursor (EGF); sb:eu639; URG; Urogastrone

Gene Symbol

EGF

Product Type

Protein

Specific Reactivity

Human

Conjugate

Unconjugated

Recombinant

Recombinant

Sequence

NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR

Activity

Determined by the proliferation of mouse Balb/c 3T3 cells. The specific activity of PeproGMP Recombinant Human EGF is 3.15 IU/ng, which is calibrated against NIBSC 91/530.

Expression System

E. coli

Purity or Quality Grade

≥ 98% by SDS-PAGE gel and HPLC analyses.