Gibco™ PeproGMP® Human SCF Recombinant Protein, PeproTech®

Recombinant Protein

Stem cell factor (SCF) is the ligand of the c-Kit oncogene and is expressed by various structural and inflammatory cells in the airways. Binding of SCF by the c-Kit receptor leads to homodimerization of the receptor and the activation of signalling pathways such as PI-3, PLC-gamma, Jak/STAT, and MAP kinase pathways. SCF expression leads to the induction of mast cell survival and the expression and release of histamine, pro-inflammatory cytokines and chemokines. The inhibition of the SCF/c-Kit pathway leads to a decrease in histamine levels, mast cell and eosinophil infiltration, IL-4 production and airway hyperresponsiveness, suggesting this pathway may be a useful therapeutic target in inflammatory diseases such as asthma. At least two isoforms of SCF are known to exist.

Accession Number

P21583

For Use With (Application)

Functional Assay

Format

Lyophilized

Formulation

Protein with no preservative

Molecular Weight (g/mol)

18.4

Name

SCF

Quantity

100 μg

Immunogen

Human SCF

Storage Requirements

-20°C or -80°C if preferred

Regulatory Status

RUO

Endotoxin Concentration

<0.1

Assays

Functional Assay

Conjugate

Unconjugated

Recombinant

Recombinant

Activity

Determined by the proliferation of TF-1 cells. PeproGMP Recombinant Human SCF has been tested against the WHO standard NIBSC 91/682.

Expression System

E. coli

Product Line

PeproGMP

Purity or Quality Grade

Purified