Invitrogen™ Phosphate Sensor

Phosphate Sensor

The Phosphate Sensor is a highly sensitive reagent for the detection of inorganic phosphate, either in real-time kinetic or end-point mode. This product is a purified form of recombinant E. coli phosphate-binding protein labeled with the fluorophore MDCC, which is sensitive to changes in its environment. Binding of inorganic phosphate by Phosphate Sensor is rapid and tight (Kd ∼ 0.1μM), resulting in a large increase in fluorescence. The Phosphate Sensor detects phosphate in the high-nanomolar to low-micromolar range, which is 100-fold more sensitive than malachite green. This reagent provides you with a sensitive assay for detecting enzymes that produce inorganic phosphate either directly or indirectly through coupled reactions, such as phosphatases, ATPases, GTPases, and phosphodiesterases.

For Use With (Equipment)

Microplate Reader

Target

GTPases, ATPases, Phosphatases, Phosphodiesterases

For Use With (Application)

Fluorescence Intensity

Assay

Phosphatase Assay

Content And Storage

Phosphate Sensor is supplied as a 500 μM solution in 10 mM Tris (pH 7.6) and 50 mM NaCl. Store product at -80°C.

Detection Method

Fluorescence

Label or Dye

MDCC

Product Type

Phosphate Sensor

Quantity

100 nmol

Format

384-well plate

Excitation/Emission

430⁄450

Readout

Kinetic

Shipping Condition

Dry Ice

For Research Use Only. Not for use in diagnostic procedures.