Phospho-RNA pol II CTD (Ser2) Monoclonal Antibody (3E10), eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Supplier:  eBioscience 14980282

Catalog No. 14-980-282

Description

Description: This 3E10 monoclonal antibody recognizes a specific phosphorylated form of RNA polymerase II subunit B1 (RPB1). It detects phosphorylated serine at amino acid position 2 (Ser2) within the C-terminal heptapeptide (YSPTSPS) repeats of RPB1. Modifications within the C-terminal domain (CTD) of RPB1 recruit RNA processing factors that govern the transcription cycle; phosphorylation at Ser2 is associated with transcriptional elongation. The 3E10 immunogen was an ovalbumin-conjugated peptide corresponding to human RPB1 CTD phosphorylated at Ser2 (YS(p)PTSPSYSPTSPSC). Applications Reported: This 3E10 antibody has been reported for use in western blot and immunocytochemistry. Applications Tested: This 3E10 antibody has been tested by western blot of human, mouse, and rat cell lysates and immunocytochemistry of human cell lines. This may be used at less than or equal to 2.0 μg/ml for western blot and less than or equal to 1.25 μg/ml for immunocytochemistry.

RNA polymerase II is the enzyme responsible for the synthesis of mRNA and many non-coding RNAs. POLR2A encodes RNA polymerase II subunit B1 (RPB1), the largest subunit of RNA polymerase II. The C-terminal domain (CTD) of RPB1 contains tandem heptapeptide repeats with the consensus sequence YSPTSPS. Phosphorylation and other modifications within this sequence influence the recruitment of factors that regulate the transcription cycle. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the CTD.

Specifications

• Antigen: Phospho-RNA pol II CTD (Ser2)
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P08775, P24928
• Gene Symbols: POLR2A
• Immunogen: Ovalbumin-conjugated peptide corresponding to human RNA polymerase subunit B1 CTD phosphorylated at Ser2 (YS(p)PTSPSYSPTSPSC)
• Quantity: 100μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG1 κ

• Applications: Immunocytochemistry, Western Blot
• Clone: 3E10
• Conjugate: Unconjugated
• Gene: POLR2A
• Gene Alias: 220kDa, AW321876, DNA polymerase alpha 1 catalytic subunit, DNA polymerase alpha 1, 180 kDa, DNA polymerase alpha 1, catalytic subunit, DNA polymerase alpha catalytic subunit, DNA polymerase alpha catalytic subunit p180, DNA polymerase alpha p180 subunit, DNA polymerase alpha subunit I, DNA polymerase epsilon catalytic subunit, DNA polymerase epsilon catalytic subunit A, DNA polymerase II subunit A, DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, DUN2, EC 2.7.7.6, G7a/Bat6Hom, hRPB220, hsRPB1, I79_002751, I79_016081, MGC75453, N0825, NSX, p180, Pol II S2p, POL2, Pola, Pola1, POLR2, POLR2A, POLRA, polymerase (DNA directed), alpha 1, polymerase (DNA directed), alpha 1, catalytic subunit, polymerase (DNA) alpha 1, catalytic subunit, polymerase (DNA-directed), alpha (70kD), Polymerase (RNA II (DNA directed), large polypeptide, polymerase (RNA) II (DNA directed) polypeptide A, polymerase (RNA) II (DNA directed) polypeptide A, 220kDa, polymerase (RNA) II subunit A, POLYMERASE II, polymerase, alpha 1, RNA, RNA POL2, RNA polymerase II 1, RNA polymerase II carboxy-terminal domain, RNA polymerase II C-terminal domain, RNA polymerase II largest subunit, RNA polymerase II subunit A, RNA polymerase II subunit B1, RNA-directed RNA polymerase II subunit RPB1, RP02, Rpb1, RPB220, RPBh1, Rpii215, RpIILS, RPO2, RPO21, Rpo2-1, RPOL2, SUA8, YNL262W
• Host Species: Rat
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 20020, 363633, 5430
• Content And Storage: 4° C, do not freeze
• Form: Liquid