Invitrogen™ pHrodo™ Antibody Labeling Kits

Perform antibody internalization, endocytosis, and phagocytosis experiments with confidence and ease using the updated pH-sensitive, brightly fluorescing dyes and ready-to-use spin columns in the pHrodo Red and Green Antibody Labeling Kits.

Elevate biopharmaceutical research with pHrodo Antibody Labeling kits, designed to characterize antibody internalization, a critical step during antibody-drug conjugate research and development. Kits provide a simple, detailed protocol and advanced purification columns to easily label and purify antibodies and other large proteins. Amine-reactive pHrodo dyes covalently bind to antibodies and other proteins to produce bright, soluble conjugates whose fluorescence increases in acidic organelles including endosomes and lysosomes. Antibody internalization can be evaluated using flow cytometry, fluorescence microscopy, and high-content screening.

The pHrodo Red Antibody Labeling Kit and pHrodo Green Antibody Labeling Kit provide the reagents needed to easily label three purified protein or antibody samples with pHrodo Red or pHrodo Green dye. These kits contain amine-reactive, pH-sensitive pHrodo Red or Green STP ester dye that covalently binds to free lysines anywhere they occur in an antibody or protein.

The dyes included in this kit are improved versions of pHrodo Red and pHrodo Green dyes. They are optimized for the creation of bright and soluble bioconjugates that are used in the study of antibody internalization, endocytosis, phagocytosis, and for development of antibody-drug conjugates. These dyes are also more soluble than the original pHrodo Red and pHrodo Green dyes, making them useful for the labeling of antibodies that may otherwise precipitate out of solution during conjugation. pHrodo Red and pHrodo Green dyes are non-fluorescent outside the cell, but fluoresce bright red or bright green as the pH of their surroundings becomes more acidic, such as those found inside of phagosomes and endosomes.

The pHrodo Red and Green Microscale Labeling Kits offer the following benefits:
• Convenience—Labeling typically takes one hour, with less than 30 minutes of hands-on time
• Versatility—Labelling can be performed on 12-150 kDa molecular weight proteins
• Customization—Create your own probes for studying antibody internalization, phagocytosis, and endocytosis

Description

Green Antibody Labeling Kit, 3 x 1 mg

For Use With (Application)

Flow Cytometry, Fluoresence Microscopy

Product Type

Labeling Kit

Quantity

3 x 1 mg Reactions

Content And Storage

Store refrigerated.

Detection Method

Fluorescence

Excitation/Emission

505/525 nm

Labeling Scale

1 mg

Product Line

pHrodo™

Chemical Reactivity

Amine

Reactive Moiety

STP Ester

Solubility

DMSO (Dimethylsulfoxide)

Label or Dye

pHrodo Green

Spacer

Mid-length, Pegylated

Color

Green

Labeling Target

Antibodies, Proteins

Flow Cytometer Laser Lines

488 nm

Label Type

pHrodo