Thermo Scientific™ 1-Step™ ABTS Substrate Solution

Ready-to-use, water-soluble peroxidase substrate tablets that yield a measurable green end-product for use in ELISA methods.

Thermo Scientific™ Pierce ABTS Substrates are tablets and a ready-to-use solution of a water-soluble peroxidase substrate that yields a measurable green end product for use in ELISA methods.

ABTS (2,2'-Azinobis [3-ethylbenzothiazoline-6-sulfonic acid]-diammonium salt) is a water-soluble HRP substrate that yields a green end product upon reaction with peroxidase. The green product has two major absorbance peaks, 410nm and 650nm. ABTS is less sensitive than OPD and TMB in ELISA applications. It is less readily oxidized, and its color development is slower (approximately 20 minutes). This may be advantageous if unacceptable background results from the use of the OPD or TMB substrates due to higher sensitivities.

Highlights:

• Excellent choice when maximum sensitivities are not required
• Less readily oxidized and color development is slower (approximately 20 minutes) than OPD and TMB

Description

Pierce 1-Step ABTS Solution

Product Type

ELISA Substrate

Detection Method

Colorimetric

Product Line

1-Step™

Substrate Properties

Chemical Substrate

Substrate Type

HRP (Horseradish Peroxidase)

Content And Storage

Store at 4°C

For Research Use Only. Not for use in diagnostic procedures.