Thermo Scientific™ Pierce™ BCA Protein Assay Reagent A

Estimate visually, or measure with a standard spectrophotometer or plate reader at 562 nm.

Thermo Scientific™ Pierce BCA Protein Assay Kit remains one of the most popular protein quantitation methods worldwide.

Additional Information and Online Ordering

• A component of the Pierce BCA Protein Assay Kit
• Kit is a two-component, high-precision, detergent-compatible assay that is used for total protein concentration determination compared to a protein standard
• Pierce BCA reagents provide accurate determination of protein concentration with most sample types encountered in protein research
• The Pierce BCA assay can be used to assess yields in whole cell lysates, affinity-column fractions, purified proteins samples, as well as to monitor protein contamination in industrial applications
• Compared to most dye-binding methods, the BCA assay is affected much less by protein compositional differences, providing greater concentration accuracy
• Estimate visually or measure with a standard spectrophotometer or plate reader at 562 nm
• Exhibits less protein-to-protein variation than dye-binding methods (Bradford)
• Unaffected by typical concentrations of most ionic and non-ionic detergents
• Assay has a 30 min. incubation; much easier and four times faster than classical Lowry methods
• Linear working range for BSA of 20 to 2000 μg/mL
• Detect down to 5 μg/mL with the enhanced protocol
• Store at room temperature

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Content And Storage

Store at room temperature.

Description

Pierce BCA Protein Assay Reagent A

Assay

BCA Assay

Detection Method

Colorimetric

Product Line

Pierce™

Product Type

Protein Quantitation Assay

Specificity

Not Target-Specific

For Use With (Application)

Solution-based Detection, Absorbance

For Use With (Equipment)

Spectrophotometer, Microplate Reader

Quantity

1 L

For Research Use Only. Not for use in diagnostic procedures.