Thermo Scientific™ Pierce™ Biotin-Fluorescein Conjugate

Description

Purified biotin and biotinylated BSA, HRP, AP or FITC for use as controls or to amplify signal in IHC via the avidin-biotin complex (ABC).

Thermo Scientific™ Pierce Biotinylated Proteins include biotin-labeled horseradish peroxidase (B-HRP), alkaline phosphatase (B-AP) and fluorescein (B-FITC) for use as controls or signal amplification in IHC via avidin-biotin complex (ABC) techniques.

Biotinylated HRP and biotinylated AP are most commonly used in immunohistochemistry (IHC) to amplify the signal of biotinylated primary antibodies using the ABC staining method. Biotinylated Fluorescein (FITC) cannot be used to polymerize and amplify the signal to the same degree as biotinylated enzymes because each fluor molecule is tagged with only one biotin molecule. However, the fluorescent variant of traditional ABC staining is possible with this unique molecule.

Highlights:

• Biotinylated enzymes – biotin-labeled horseradish peroxidase (B-HRP), alkaline phosphatase (B-AP) and beta-galactosidase (B-bGal) for use in avidin-biotin complex (ABC) staining

• Biotinylated FITC – amplify the fluorescein signal of biotinylated primary antibodies by using avidin or streptavidin plus biotinylated fluorescein (B-FITC)

• Biotinylated controls – use biotinylated bovine serum albumin (B-BSA) or any one of these molecules as positive controls in biotin quantitation methods

Specifications

• Conjugate: Classic Dyes, Biotin and Analogs
• Description: Pierce Biotin-Fluorescein Conjugate
• Quantity: 5 mg
• Content And Storage: Store at 4°C, protected from moisture. Shipped at room temperature.
• Excitation/Emission: 494/518
• Solubility: DMF (Dimethylformamide)
• Form: Solid
• Product Line: Pierce™
• Product Type: Biotin Conjugate (Fluorescein)

For Research Use Only. Not for use in diagnostic procedures.