Thermo Scientific™ BS(PEG)5 (PEGylated bis(sulfosuccinimidyl)suberate)

Crosslink amines to amines with these long (21.7A and 35.8A) crosslinkers composed of NHS-ester groups and 5-unit or 9-unit PEG spacer arms that enhance protein solubility.

Thermo Scientific™ Pierce BS(PEG)n Crosslinkers include BS(PEG)5 and BS(PEG)9, which are bis-succinimide ester-activated PEG compounds for crosslinking between primary amines in proteins and other molecules.

BS(PEG)5 is a bis-succinimide ester-activated PEG compound used for crosslinking between primary amines (—NH2) in proteins and other molecules. The N-hydroxysuccinimide ester (NHS) groups at either end of the PEG5 spacer react specifically and efficiently with lysine and N-terminal amino groups at pH 7-9 to form stable amide bonds.

Thermo Scientific No-Weigh products are specialty reagents provided in a pre-aliquoted format. The pre-weighed packaging prevents the loss of reagent reactivity and contamination over time by eliminating the repetitive opening and closing of the vial. The format enables use of a fresh vial of reagent each time, eliminating the hassle of weighing small amounts of reagents and reducing concerns over reagent stability.

Features of BS(PEG)5:

• Reactive groups: NHS ester (both ends)
• Reactive towards: amino groups (primary amines)
• NHS esters react with—NH2 groups at pH 7-9, forming stable amide bonds
• Water-soluble; compare to non-pegylated BS3 (also soluble) (Cat. No. 21580) and DSS (water-insoluble) (Cat. No. 21655)
• Membrane-impermeable, allowing for cell surface labeling
• Irreversibly crosslink proteins or peptides by flexible PEG spacer arms
• Polyethylene glycol spacer arms help maintain conjugate solubility
• Pure compound with defined structure and molecular weight, ensuring reproducible protein-modification effects
• PEG spacer provides unique advantages, including increased stability, reduced tendency toward aggregation and reduced immunogenicity
• Ideal for small molecule or peptide conjugations

Alternative Names for BS(PEG)n:

• Bis-NHS-dPEG
• NHS-PEG-NHS
• BS(PEO)5
• Bis(NHS)PEO5
• BS-PEO9
• dPEG NHS ester
• Bis-succinimide ester PEG 400

Product References

• Crosslinker Application Guide -- search for recent literature references for this product
• Bioconjugate Techniques, Academic Press (Cat. No. 20036)

Related Products

• BS(PEG)9 (PEGylated bis(sulfosuccinimidyl)suberate) (Cat. No. 21582)
• BS3 (bis(sulfosuccinimidyl)suberate) (Cat. No. 21580)

Chemical Reactivity

Amine-Amine

Cleavable

No

Description

BS(PEG)5

Molecular Weight (g/mol)

532.5

PEGylated

Yes

Spacer Arm Length

21.7 Å

Content And Storage

Upon receipt store desiccated at -20°C.

Cell-Permeable

No

Shipping Condition

Wet Ice

Product Line

No-Weigh™, Pierce™

Labeling Method

Chemical Labeling

Crosslinker Type

Homobifunctional

Reactive Moiety

Sulfo-NHS Ester

Spacer

Medium (10 to 30 Å)

Form

Powder

Quantity

10 x 1 mg

Solubility

Water

Format

Standard, Single use

Water-Soluble

Yes

Product Type

Crosslinker

For Research Use Only. Not for use in diagnostic procedures.