Thermo Scientific™ Pierce™ Conjugate Purification Kit

Gently purify enzyme-conjugated antibodies from unreacted horseradish peroxidase (HRP) or alkaline phosphatase (AP) after labeling.

Thermo Scientific™ Pierce Conjugate Purification Kit is a unique affinity chromatography system for purifying horseradish peroxidase (HRP) or alkaline phosphatase (AP) conjugated antibodies from unreacted enzyme after labeling.

The metal chelate ligand on the column binds the enzyme conjugates while allowing free enzyme to pass through.

Highlights:

• Reduced background for blotting and ELISA
• Fewer washes needed to remove background
• Increased sensitivity of assay system with more active reagent
• Improved signal:noise ratio of conjugate

Includes:

Immobilized Iminodiacetic acid 1 × 2mL column, Polyacrylamide Desalting Column 1 × 10mL column, FreeZyme Column Activator Buffer 25mL, FreeZyme Elution Buffer 35mL, BupH™ Tris Buffered Saline Pack two each, BupH Phosphate Buffered Saline Pack one each, Activity Preservation Solution 1mL

Product Type

Conjugate Purification Kit

Quantity

1 kit

Sufficient For

10 purifications, each of 0.5 to 50 mg of enzyme-conjugated antibody

Content And Storage

Sufficient For: 10 purifications, each of 0.5 to 50 mg of enzyme-conjugated antibody

IDA Agarose Column, 2 mL, 1 column

Polyacrylamide Desalting Column, 10 mL, 1 column

Column Activator Buffer, 25 mL

Elution Buffer, 35 mL

TBS Packs (each makes 500 mL), 2 packs

PBS Pack (makes 500 mL), 1 pack

Activity Preservation Solution, 1 mL

Store kit at 4°C.

Labeling Scale

0.5 to 3 mg

Product Line

Pierce™

Label or Dye

HRP (Horseradish Peroxidase)

Labeling Target

IgG

For Research Use Only. Not for use in diagnostic procedures.