Thermo Scientific™ Pierce™ Bradford Protein Assay Kit

Brown-to-purple (A595nm), ready-to-use, classic reducing agent-compatible assay reagent to measure total protein concentration vs. protein standard.

Measure protein concentration using a stable formulation of the original Bradford reagent described in the literature with Thermo Scientific™ Pierce Coomassie Protein Assay Kit.

The Pierce Coomassie Protein Assay Kit is a ready-to-use formulation of the popular assay reagent originally described by Bradford in 1976. When mixed with a protein solution, the acidic Coomassie-dye reagent changes color from brown to blue in proportion to the amount of protein present in the sample. Protein determinations are made by comparison to the color response of protein assay standards, usually prepared as a series of known dilutions of bovine serum albumin (BSA) or bovine gamma globulin (BGG). The kit includes Coomassie Protein Assay Reagent and a package of Albumin Standard Ampules. The simple procedure is adaptable to nearly any volume scale, including test tubes, cuvets and microplates.

Highlights:

• Bradford reagent – stable, ready-to-use kit of the classical Bradford assay reagent

• Colorimetric – measure with a standard spectrophotometer or plate reader (595nm)

• Easy to use – single reagent; no working reagent preparation required

• Fast – almost immediate color development; add, mix and read results

• Broad range – detects protein concentration in the range 1 to 1500μg/mL

• Flexible – microplate and cuvet protocols provided with the instructions and adaptable to several target working ranges

Includes:

Coomassie Protein Assay Reagent and 10 ampules of Albumin Standard

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Content And Storage

Sufficient For:

• 630 tube assays or 3800 microplate assays
• Pierce Bradford Assay Reagent, 950 mL
• Albumin Standard ampules, 2 mg/mL, 10 x 1 mL

Store Bradford Protein Assay Reagent at 4°C. Store unopened Albumin Standard ampules at room temperature.

Assay

Bradford Assay

Detection Method

Colorimetric

Product Line

Pierce™

Product Type

Protein Quantitation Assay

Specificity

Not Target-Specific

Sufficient For

630 Tube Assays or 3800 Microplate Assays

For Use With (Application)

Solution-based Detection, Absorbance

For Use With (Equipment)

Spectrophotometer, Microplate Reader

Quantity

950 mL

For Research Use Only. Not for use in diagnostic procedures.