Thermo Scientific™ Pierce™ Cypridina Luciferase Flash Assay Kit

Assay regulatory element transcriptional activity in mammalian cell culture media and whole cell lysate with this highly sensitive flash-based luciferase kit.

The Thermo Scientific™ Pierce Cypridina Luciferase Flash Assay Kit provides researchers with a highly sensitive assay for transcriptional activity of regulatory elements in mammalian cell culture media and whole cell lysate.

This Flash Assay Kit contains reagents for measuring the activity of Cypridina luciferase in mammalian cell culture media and lysates. When used with Thermo Scientific Cypridina Luc Vectors, the kit provides an extremely sensitive bioluminescent reporter assay system for secreted or intracellular detection of promoter or pathway activity. Cypridina luciferase is highly secreted into the cell culture media, allowing for live cell monitoring of reporter activity. The signal produced by Cypridina is considerably greater than signal from either firefly or Renilla luciferases assayed under similar conditions.

Highlights:

• Sensitive – high sensitivity allows utilization of smaller numbers of cells
• Cost effective – sensitive assays result in decreased reagent consumption
• Secreted luciferase – allows real-time assays and kinetic studies without destroying cells
• Ideal for co-transfection – uses a different substrate than Renilla, Gaussia, or firefly luciferases
• Compatible – assay reagents compatible with other Cypridina or Vargula luciferases
• Time-saving – assays using secreted luciferases require minimal sample handling
• Automation-friendly – amenable to high throughput screens
• Convenient – contains a universal cell lysis buffer and optimized flash assay reagent
• Safe – allows one to perform non-radioactive assays

Includes:

Cell lysis buffer, reaction buffer and substrate

Requires:

Cypridina luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers.

Recommended for:

Promoter studies for analyzing cis- regulatory elements and trans-acting factors; Drug screening; siRNA and miRNA screening; Multiplexed assays to study off-target effects; Secretory pathway / protein localization reporter assays; Signal transduction pathway analysis; RNA splicing studies

Type

Assay Kit

Target

Luciferase, Cypridina Luciferase

Quantity

1000 Reaction Kit

Content And Storage

• Cypridina Flash Assay Buffer, 5 mL, store at 4°C
• Vargulin (1X), 0.5 mL, store at -20°C
• 2X Cell Lysis Buffer, 6 mL, store at room temperature

Upon receipt store kit at -20°C or store individual components as indicated above.

Assay

Reporter Enzyme, Luciferase Reporter Assay

Compatible Cells

Mammalian Cells

Detection Method

Bioluminescence

For Use With (Equipment)

Luminometer (Microplate)

Format

384-well plate, 96-well plate

Label Type

Enzyme Labeled

Substrate Properties

Chemical Substrate

Technique

Enhanced Chemiluminescence

Substrate Type

Luciferase Substrate

Product Line

Pierce™

Substrate

Vargulin (Cypridina Luciferin)

For Research Use Only. Not for use in diagnostic procedures.