Thermo Scientific™ Pierce™ Cypridina Luciferase Glow Assay Kit

Obtain bright and stable bioluminescence signal (half-life greater than 1 hour) in the presence of Cypridina luciferase reporters.

The Thermo Scientific™ Pierce Cypridina Luciferase Glow Assay Kit provides an extremely bright and stable bioluminescence signal (half-life greater than 1 hour) in the presence of Cypridina luciferase reporters.

This Pierce Luciferase Glow Assay Kit contains reagents for measuring Cypridina luciferase activity in mammalian cell culture media and lysates. When used with Thermo Scientific Cypridina Luc Vectors, the kit provides an extremely sensitive bioluminescent reporter assay system for secreted or intracellular detection of luciferase activity. The light output generated by the luciferase reaction can be correlated to the amount of luciferase protein produced, which in turn is proportional to the activity of the promoter driving the luciferase expression. The kit can be used to detect the activity of any Cypridina luciferases or derivatives that use Vargulin as a substrate.

Highlights:

• Sensitive – highly sensitive detection of Cypridina luciferase activity

• Secreted luciferase – allows real-time assays and kinetic studies without destroying cells

• Stable – reagents provide increased signal stability compared to flash assays

• Simple – does not require a luminometer with injectors

• Compatible – assay reagents compatible with other Cypridina or Vargula luciferases

• Automation-friendly – amenable to high throughput assays

• Convenient – contains a universal cell lysis buffer and optimized glow assay reagent

• Safe – allows one to perform non-radioactive assays

Includes:

Cell lysis buffer, reaction buffer and substrate

Requires:

Cypridina luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers

Recommended for:

Promoter studies for analyzing cis- regulatory elements and trans-acting factors; Drug screening; siRNA and miRNA screening; Multiplexed assays to study off-target effects; Secretory pathway / protein localization reporter assays; Signal transduction pathway analysis; RNA splicing studies

Type

Assay Kit

Target

Luciferase, Cypridina Luciferase

Quantity

1000 Reaction Kit

Content And Storage

• Cypridina Glow Assay Buffer, 5 mL, store at 4°C
• Vargulin (1X), 0.5 mL, store at -20°C
• 2X Cell Lysis Buffer, 6 mL, store at room temperature

Upon receipt store kit at -20°C or store individual components as indicated above.

Assay

Reporter Enzyme, Luciferase Reporter Assay

Compatible Cells

Mammalian Cells

Detection Method

Bioluminescence

For Use With (Equipment)

Luminometer (Microplate)

Format

384-well plate, 96-well plate

Label Type

Enzyme Labeled

Substrate Properties

Chemical Substrate

Technique

Enhanced Chemiluminescence

Substrate Type

Luciferase Substrate

Product Line

Pierce™

Substrate

Vargulin (Cypridina Luciferin)

For Research Use Only. Not for use in diagnostic procedures.