Thermo Scientific™ Pierce™ EDC, No-Weigh™ Format

Immobilize or conjugate proteins using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (CAS 25952-53-8), a water-soluble, carboxyl-to-amine crosslinker.

Thermo Scientific™ Pierce EDC is a water-soluble carbodiimide crosslinker that activates carboxyl groups for spontaneous reaction with primary amines, enabling peptide immobilization and hapten-carrier protein conjugation.

EDC is the abbreviation for 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride, a crosslinker that has been used in diverse applications such as forming amide bonds in peptide synthesis, attaching haptens to carrier proteins to form immunogens, labeling nucleic acids through 5Æphosphate groups and creating amine-reactive NHS-esters of biomolecules. EDC reacts with a carboxyl to form an amine-reactive O-acylisourea intermediate. If this intermediate does not encounter an amine, it will hydrolyze and regenerate the carboxyl group. In the presence of N-hydroxysulfosuccinimide (Sulfo-NHS, Part No. 24510), EDC can be used to convert carboxyl groups to amine-reactive Sulfo-NHS esters.

Highlights:

• Zero-length crosslinker
• Numerous chemical conjugates can be synthesized via available –COOH and –NH2 groups
• Amide bond formed provides a neutral linkage, which is ideal for preparing peptides and antigens
• Adding Sulfo-NHS (Part No. 24510) enhances the coupling reaction at physiological pH conditions
• Reactive groups: carbodiimide
• Reactive toward: carboxyl and amino groups

Chemical Reactivity

Amine-Carboxyl

Cleavable

No

Description

EDC

Molecular Weight (g/mol)

191.7

PEGylated

No

Spacer Arm Length

0.0 Å

Content And Storage

Upon receipt store desiccated at -20°C.

Cell Permeability

No

Shipping Condition

Wet Ice

Product Line

Pierce™

Labeling Method

Chemical Labeling

Crosslinker Type

Heterobifunctional

Reactive Moiety

Carbodiimide

Spacer

Short (<10 Å)

Form

Powder

Quantity

5 g

Solubility

Water

Format

Standard, Single-use, Premium-grade

Water Soluble

Yes

Product Type

Crosslinker

For Research Use Only. Not for use in diagnostic procedures.