Thermo Scientific™ Fluoraldehyde™ o-Phthaldialdehyde Crystals (OPA)

Measure peptides or amino acids (Ex 340nm, Em 455nm) with phthalaldehyde, a fluorescent, amine-reactive, HPLC pre- or post-column labeling reagent.

Thermo Scientific™ Pierce Fluoraldehyde (OPA) is o-phthalaldehyde, a highly sensitive fluorescent pre- or post-column derivatization reagent for peptide or amino acid detection and quantitation in HPLC.

Fluoraldehyde Crystals are a specially purified, fluorogenic-grade preparation of o-phthalaldehyde (OPA). This amine-reactive compound is primarily used for high-sensitivity, fluorescent detection of primary amines, including amino acids, peptides, proteins and polyamines in pre- or post-column chromatographic effluents.

Highlights:

• For pre- or post-column amino acid derivatization for fluorescent detection and quantitation

• Reacts with all primary amine-containing analytes to yield fluorescent derivatives (ex/em = 340nm/455nm)

• Provides an accurate measure of both composition and absolute protein-peptide content

• Ideal for work with recombinant proteins and synthetic peptides

• Can be used for fluorescent protein or peptide assay

• Can be prepared as a stable aqueous solution in borate (see instructions)

Reagent Properties:

• Can be prepared as a stable aqueous solution in borate (see instructions)

• Alternative names: Fluoraldehyde Crystals, OPA

• Chemical name: ortho-Phthalaldehyde

• Reactive toward: Primary amines (–NH2)

• Chemical formula: C8H6O2

• CAS number: 643-79-8

• Molecular weight: 134.12

• Form: Pale yellow, free-flowing crystals

Product Type

o-Phthaldialdehyde

Chemical Reactivity

Amine

Product Line

Fluoraldehyde™

Content And Storage

Store at room temperature.

Label or Dye

OPA (o-phthaldialdehyde)

Detection Method

Fluorescence

Quantity

5 g

Form

Solid

For Research Use Only. Not for use in diagnostic procedures.