Thermo Scientific™ Pierce™ Glycoprotein Carbohydrate Estimation Kit

Measure protein glycosylation as the percent of purified protein mass compared to included glycoprotein standards; uses periodic acid Schiff-base reagents (PAS).

Thermo Scientific™ Pierce Glycoprotein Carbohydrate Estimation Kit enables the amount of protein glycosylation to be measured as the percent of total purified protein mass.

The Glycoprotein Carbohydrate Estimation Kit includes six purified glycoprotein standards and the required assay reagents to effectively estimate the amount of oxidizable glycosylation (% carbohydrate by weight) of purified protein samples. Sugar groups in the glycoprotein sample are first oxidized with sodium meta-periodate to produce detectable aldehyde groups. Then the sample is reacted with the colorimetric Glycoprotein Detection Reagent. Absorbance at 550nm of the resulting purple reaction product is measured with a spectrophotometer or plate reader. Finally, the carbohydrate content in the glycoprotein is calculated by comparison to results from the five glycoprotein standards that are included in the kit.

Highlights:

• Qualitative – easily identifies purified proteins as glycoproteins or samples as contaminated with sugars

• Semi-quantitative – estimates the percent carbohydrate content (w/w) of purified glycoprotein by comparison to the included set of glycoprotein standards

• Simple procedure – completed in less than 75 minutes; instructions include microplate and test tube protocols

• Adaptable – standard curve format allows for the design of alternative tests for aldehydes and carbohydrate components

Includes:

Sodium periodate, assay buffer, detection reagent and a 5-vial set of glycoprotein standards

Recommended for:

Determine if a purified polyclonal antibody or other protein is glycosylated before attempting to perform carbohydrate-based conjugation or immobilization chemistries with hydrazide reagents; Characterize and classify purified protein fractions from an affinity procedure; Assess sugar and carbohydrate contamination in non-glycosylated protein samples

Content And Storage

Sufficient For: 60 tube assays or 250 microplate assays
• Sodium meta-Periodate, 500 mg
• Pierce Glycoprotein Detection Reagent, 500 mg
• Glycoprotein Assay Buffer, 250 mL
• Glycoprotein Standards Set, 5-protein set

Description

Pierce Glycoprotein Carbohydrate Estimation Kit

Assay

Glycoprotein Carbohydrate Estimation

Detection Method

Colorimetric

Product Line

Pierce™

Product Type

Glycoprotein Carbohydrate Estimation

Specificity

Not Target-Specific

Sufficient For

60 Tube Assays or 250 Microplate Assays

For Use With (Application)

Solution-based Detection, Absorbance

For Use With (Equipment)

Spectrophotometer, Microplate Reader

Quantity

500 mg

For Research Use Only. Not for use in diagnostic procedures.