Thermo Scientific™ Pierce™ High Capacity Endotoxin Removal Spin Columns, 0.25 mL

Description

Remove endotoxins (lipopolysaccharides or LPS) from samples before mass spectrometry using spin columns of poly(e-lysine) ligand on beaded cellulose.

Reduce endotoxin levels in protein samples by greater than 99% in one hour while maximizing protein recovery with Thermo Scientific™ Pierce High Capacity Endotoxin Removal Resin.

The Pierce High Capacity Endotoxin Removal Resin combines porous cellulose beads and an FDA-approved food preservative, poly(epsilon-lysine), as an affinity ligand to selectively bind endotoxins. Endotoxin levels in protein samples are reduced by greater than 99% in as little as 1 hour using our spin cup format, and protein recovery is ≥85%. The modified polylysine affinity ligand eliminates the toxicity concerns associated with alternative technologies using polymixin B ligands and sodium deoxycholate buffers. Pierce High capacity Endotoxin Removal Resin is available as a slurry to pack a custom column that can be re-used at least 5 times with no loss in endotoxin removal efficiency. This resin is also available in a new single-use spin column format optimized for different sample volumes.

Highlights:

• High capacity bind up to 2,000,000 EU/mL to eliminate at least 99% of endotoxins

• Durable re-use resin up to 10 times with no loss in performance

• Selective recover at least 85% of your protein sample

• High performance compatible with FDA guidelines by reducing final EU concentration to less than 5 EU/mL

• Fast our new spin cup format enables endotoxin depletion within 1 hour

• Cleansingle-use spin columns avoid cross contamination of samples

• Optimized spin cups are optimized for different sample volumes

• Economical large-volume discounts available

Requires:

Endotoxin-free water

Recommended for:

Eliminate endotoxins from protein or antibody samples prior to cell transfection or animal injection

Specifications

• Capacity (Metric): Up to 2,000,000 EU/mL
• Formulation: 5mL-capacity centrifuge columns containing 0.25mL resin
• Content And Storage: Upon receipt store at 4°C.
• Form: Liquid
• Product Line: Pierce™
• Quantity: 5 Columns
• Target: Endotoxin Removal
• Type: Spin Column
• Stationary Phase: Proprietary Ligand
• Format: Spin Column
• Column Type: Affinity, Cellulose Resin

For Research Use Only. Not for use in diagnostic procedures.