Thermo Scientific™ Pierce™ High Capacity Streptavidin Agarose

Bind biotinylated proteins and other ligands with a wide selection of high-quality agarose and acrylamide beads and columns of recombinant streptavidin biotin-binding protein.

Pierce Streptavidin Agarose and UltraLink Resins supply immobilized streptavidin biotin-binding protein in different resin formats, binding capacities and package sizes to suit nearly any immunoprecipitation or affinity purification procedure involving biotinylated molecules. The products use a recombinant form of streptavidin with a mass of 53 kDa and a near-neutral pI. The resins are prepared by covalently coupling streptavidin to the porous agarose or UltraLink Biosupport using efficient and stable chemistries, resulting in Streptavidin Agarose and UltraLink Resins that are leach-resistant and stable at pH 2-11. The products are excellent choices for a variety of small- or large-scale affinity purification applications involving biotinylated macromolecules, including separation of biotinylated molecules from samples and immunoprecipitation of antigens using biotin-labeled antibodies.

Highlights:

• Proven coupling chemistries – the methods used to prepare these resins are superior to typical cyanogen bromide (CNBr) immobilization, providing high functionality, low nonspecific binding and excellent leach-resistance

• Versatile resins – Agarose (polysaccharide-based) and UltraLink™ Biosupport (acrylamide-based) resins provide two different options for particular experimental conditions and are compatible with gravity-flow, spin and FPLC methods

• Available in multiple formats – choose from two resin types, regular or high capacity preparations, and small or large-scale package sizes (bulk sizes are also available)

• Superior performance – High Capacity Resin has 2 to 3 times higher binding capacity than alternative supplier products, allowing use of small amounts of resin for experiments

Item-Specific Features:

• Agarose Resins are crosslinked 6% beaded agarose
• UltraLink Resins beaded acrylamide support (UltraLink Biosupport)
• Plus and High Capacity Resins have higher loading (i.e. density) of immobilized streptavidin protein than the regular forms

Recommended for:

Purification of membrane antigens in conjunction with biotinylated monoclonal antibodies; Cell-surface labeling with biotinylation reagents, followed by precipitation with immobilized streptavidin; Purification of cell-surface glycoproteins using biotinylated Concanavalin A; Recovery of single-stranded DNA for dideoxy sequencing

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Capacity (Metric)

10 mg Biotinylated BSA Binding per mL Resin

Content And Storage

Upon receipt store at 4°C. Do not freeze the resin. Product is shipped at ambient temperature.

Particle Size

45 to 165 μm

Product Line

Pierce™

Quantity

10 mL

Protein Form

Recombinant

Target

Biotin-Tagged Molecule

Type

Agarose

Stationary Phase

Streptavidin

Sufficient For

Binding ≥/= 100 μg Biotin/mL Resin (≥/= 10 mg Biotinylated BSA/mL Resin)

Format

Bottle

Column Type

Affinity, Agarose Resin

For Research Use Only. Not for use in diagnostic procedures.