Thermo Scientific™ Pierce™ Immunodiffusion Plates, Multiple Pattern

Identify and characterize antibody-antigen binding interactions with these standard Ouchterlony gel plates with patterns of six wells around one well.

Thermo Scientific™ Pierce Immunodiffusion Plates are standard Ouchterlony gel plates containing patterns of six wells around one well for studying and characterizing antibody-antigen binding interactions.

Immunodiffusion (ID) is a classic technique for detecting antibody-antigen interactions based on the theory of double diffusion originally described by Oudin¹ and Ouchterlony.² Antigens and antibodies are placed into separate wells that are cut into a gel matrix and allowed to diffuse towards each other. If the reaction is positive, a precipitate forms that appears as an opaque line. The precipitation reaction occurs when the antigen and antibody concentrations are combined at near equivalent proportions.

When multivalent antigens combine with divalent antibodies in solution, three-dimensional lattices are formed that aggregate and precipitate. The amount of precipitate varies in proportion to the concentration of the antigen and antibody. At equivalent or optimal proportions almost all the antigen and antibody will precipitate. If there is an excess of antibody, the complexes formed with the antigen are insoluble. When there is an excess of antigen, the precipitate has a tendency to dissolve caused by the formation of soluble complexes.

Highlights:

• Gelling agent contains precipitin brighteners and diffusion enhancers that help visualize and speed the interaction process, respectively
• Good precipitin bands from all species (including rabbit)
• Gels can be washed, dried and stained for a permanent record

Assay

Immunodiffusion (ID)

Content And Storage

Upon receipt store at 4°C. Plates are shipped at ambient temperature. Do not freeze plates or allow them to become dry.

Detection Method

Precipitate

Color

Clear

Type

Multi-Well Plate

Volume (Metric) Working

22 μL

Well Count

6

Product Line

Pierce™

Quantity

6 plates

Target Specificity

Not Target-Specific

Technique

Immunodiffusion (ID)

Height (Metric)

2.8 mm

Diameter (Metric)

3.2mm

For Research Use Only. Not for use in diagnostic procedures.