Thermo Scientific™ Pierce™ Iodination Beads

Description

Easily and gently label (iodinate) proteins and peptides with iodine-125 using reagent-coated polystyrene beads that can be removed following reaction.

Thermo Scientific™ Pierce Iodination Beads are 3mm diameter polystyrene beads that are coated with an oxidizing reagent, which provides efficient activation of iodine-125 for protein or peptide iodination procedures.

Highlights:

• Derivatized, uniform, nonporous polystyrene beads
• Radioiodide incorporation as high as 99%; labeled protein recovery >90%
• Functions over a broad pH and temperature range
• Fast and easy to use: iodinations complete in 2 to 15 minutes
• Reaction stopped by simply removing beads from reaction mixture with tweezers or pasteur pipet; no reducing agent necessary to terminate reaction

Additional Features:

• Iodinates in the presence of azides, detergents, urea and high salt
• Allows efficient iodination of cell membrane surface proteins
• More gentle method for iodination than soluble chloramine-T because there is no contact between the protein and the immobilized oxidizing agent¹
• Highly reproducible iodinations
• Can be used to quantitatively iodinate histidine at pH 8.22

Specifications

• Product Type: Iodination Bead
• Quantity: 50 Beads
• Content And Storage: Upon receipt store product desiccated at 4°C. Avoid exposing beads to moisture or reducing agents. Product is shipped at ambient temperature.
• Product Line: Pierce™
• Label or Dye: Iodine-125
• Label Type: Radioisotope

For Research Use Only. Not for use in diagnostic procedures.