Thermo Scientific™ EZ-Link™ Maleimide Activated Horseradish Peroxidase

Activated horseradish peroxidase (HRP) enzyme and kit to label proteins, peptides or other ligands via sulfhydryl groups (reduced cysteines) to make HRP conjugates.

Thermo Scientific™ Pierce Maleimide-Activated HRP Conjugation Kit can be used to directly prepare HRP conjugates from proteins, peptides or other ligands that contain sulfhydryl groups, such as reduced cysteines.

The Thermo Scientific Maleimide Activated Horseradish Peroxidase (HRP) Kit can be used to directly prepare HRP conjugates from proteins, peptides or other ligands that contain a free sulfhydryl group. This kit eliminates the first step of the two-step maleimide method, making conjugate preparation much simpler. The kit is supplied with a stable, Maleimide-activated horseradish peroxidase. HRP conjugates can be purified by gel filtration chromatography, ultrafiltration or dialysis, depending upon the size of the conjugated protein.

Highlights:

• Prepare enzyme conjugates from proteins that contain a free sulfhydryl
• Includes 2-MEA to generate free sulfhydryls from disulfide bonds
• Includes SATA to add free sulfhydryls to lysine residues

Chemical Reactivity

Thiol

Quantity

5 mg

Solubility

Water

Reactive Moiety

Maleimide

Content And Storage

Note: Total weight is ∼25mg as a result of buffer components and salts lyophilized along with the activated protein

Storage: Upon receipt store product at 4°C. Product is shipped at ambient temperature.

Formulation

Activated horseradish peroxidase lyophilized from PBS

Form

Solid

Product Line

EZ-Link™

Label Type

Enzyme Labeled

Label or Dye

HRP (Horseradish Peroxidase)

For Research Use Only. Not for use in diagnostic procedures.