Thermo Scientific™ Pierce™ Modified Lowry Protein Assay Kit

Clear-to-purple (A750nm), 2-component, folin phenol- and copper-based reagent system to measure total protein concentration vs. protein standard.

Thermo Scientific™ Pierce Modified Lowry Protein Assay is a classical, well-characterized copper-based method for measuring protein concentration.

Additional Information and Online Ordering

The Modified Lowry Protein Assay Kit combines a stabilized formulation of the original Lowry Reagents and the essential Folin-Ciocalteu Phenol reagent in a complete kit for accurately determining protein concentration in a variety of samples types. Although newer protein assay methods provide greater speed and convenience, the Lowry method remains a popular, accurate, and useful option for many applications.

Highlights:

• Popular method – widely cited in protein research literature

• Colorimetric – measure with a standard spectrophotometer or plate reader (750nm)

• Stable – uses a modified cupric sulfate-tartrate reagent that is stable at room temperature

• Large range – exhibits good linearity in the range 1 to 1500μg/mL (tested with BSA protein)

• Convenient – microplate and cuvet protocols provided with the instructions

Includes:

Modified Lowry Protein Assay Reagent, Folin-Ciocalteu Phenol Reagent (2N), and ampules of Albumin Standard (2mg/mL)

Content And Storage

Sufficient For: 480 tube assays or 2400 microplate assays

• Modified Lowry Protein Assay Reagent, 480 mL
• 2N Folin-Ciocalteu Phenol Reagent, 50 mL
• Albumin Standard Ampules, 2 mg/mL, 10 x 1 mL

Store at 4°C.

Assay

Lowry Assay

Detection Method

Colorimetric

Product Line

Pierce™

Product Type

Protein Quantitation Assay

Specificity

Not Target-Specific

Sufficient For

480 Tube Assays or 2400 Microplate Assays

For Use With (Application)

Solution-based Detection, Absorbance

For Use With (Equipment)

Spectrophotometer, Microplate Reader

Quantity

530 mL

For Research Use Only. Not for use in diagnostic procedures.