Thermo Scientific™ Pierce™ Mouse IgG1 Fab and F(ab')2 Preparation Kit

Efficiently digest mouse IgG1 antibodies into Fab or F(ab')2 fragments with immobilized ficin protease.

Thermo Scientific™ Pierce Mouse IgG₁; Fab and F(ab')₂ Preparation Kits use immobilized ficin protease to efficiently digest mouse IgG₁ into Fab or F(ab')₂ fragments, depending on the chosen reaction conditions.

The kits use ficin protease to enzymatically digest the Fc portion of whole IgG to yield Fab or F(ab')₂ fragments. Because the ficin protease is supplied in immobilized form as beaded agarose resin, the digestion reaction is easily stopped by removing the resin from the IgG solution; the result is digest products that are enzyme-free. An optimized procedure and high-performance desalting and Protein A spin columns make these kits easy to use.

Highlights:

• Enzyme-free digestion products – immobilized ficin (beaded agarose resin) provides for control of the digestion reaction and complete removal of resulting antibody fragments from the protease

• Optimized for mouse IgG₁ – the kit procedure is specifically optimized for monoclonal mouse IgG₁ antibodies, providing the best possible yield for this antibody subclass

• High capacity – kit provides for digestion and purification from as much as 4mg of IgG at a time. Use the standard size kit for fragmenting 0.25 to 4mg IgG, and the micro kit for 25 to 250μg IgG

• Complete and easy to use – kits include all reagents, spin columns and accessories needed to prepare and purify antibody fragments

Includes:

Ficin agarose resin, cysteine, digestion buffer, Protein A columns with binding and elution buffers, spin desalting columns and accessories

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Quantity

2.5 mL

Product Line

Pierce™

Content And Storage

Sufficient For: 10 antibody samples, each containing 0.25 to 4 mg IgG
• Pierce Ficin Agarose, 2.5 mL
• Cysteine-HCl, 1 g
• Mouse IgG1 Digestion Buffer, 120 mL
• NAb Protein A Plus Spin Column, 1 mL, 1 column
• Protein A Binding Buffer, 120 mL
• IgG Elution Buffer, 120 mL
• Zeba Desalt Spin Columns, 7K MWCO, 2 mL, 10 columns
• Spin Columns, 0.8 mL, 10 columns
• Microcentrifuge tubes, 2 mL, 30 tubes

Store at 4–8°C.

For Research Use Only. Not for use in diagnostic procedures.