Thermo Scientific™ NHS-Azide

Activated azido compounds to derivatize proteins or amine surfaces for selective conjugation, detection or purification by phosphine-modified probes.

Thermo Scientific™ Pierce NHS-Azide reagents are amine-reactive compounds that can be used to derivatize primary amines of proteins or amine-coated polymer surfaces for ligation to phosphine-modified molecules.

These NHS-ester compounds react to form covalent bonds with primary amines (e.g., side chain of lysine residues or aminosilane-coated surfaces). The azide (N3) group reacts with phosphine-labeled molecules by a mechanism known as Staudinger chemistry, enabling efficient and specific conjugation of derivatized molecules in biological samples. In combination with different phosphine-activated compounds, these NHS-Azide reagents facilitate a variety of labeling and crosslinking experimental strategies for investigating protein interactions and cellular pathways.

Highlights:

• Soluble – reagents easily dissolve in water-miscible solvents for subsequent dilution in aqueous reaction mixtures with cell lysates and other biological samples

• Compatible – reaction chemistry occurs effectively in simple buffer conditions; requires no accessory reagents such as copper or reducing agents

• Specific – NHS esters are specific for covalent attachment to primary amines (side chain of lysine or N-terminus of polypeptides)

 

• Chemoselective – azide and phosphine groups do not react or interfere with components of biological samples but conjugate to one another with high efficiency

• Pegylated – choose the very short NHS-Azide or one of the two varieties whose spacer arms contain medium (4-unit) or long (12-unit) polyethylene glycol (PEG) groups for increased solubility and reach

Chemical Reactivity

Amine-Phosphine

Cleavable

No

Description

NHS-Azide

Molecular Weight (g/mol)

198.14

PEGylated

No

Spacer Arm Length

2.5 Å

Content And Storage

Upon receipt store at -20°C protected from light and moisture.

Cell Permeability

Not determined

Shipping Condition

Ambient

Product Line

Pierce™

Labeling Method

Chemical Labeling

Crosslinker Type

Heterobifunctional

Reactive Moiety

Amine, Azide, NHS Ester

Spacer

Short (<10 Å)

Form

Powder

Quantity

10 mg

Solubility

DMF, DMSO

Format

Standard

Water Soluble

No

Product Type

Crosslinker

For Research Use Only. Not for use in diagnostic procedures.