Thermo Scientific™ NHS-Azide

Description

Activated azido compounds to derivatize proteins or amine surfaces for selective conjugation, detection or purification by phosphine-modified probes.

Thermo Scientific™ Pierce NHS-Azide reagents are amine-reactive compounds that can be used to derivatize primary amines of proteins or amine-coated polymer surfaces for ligation to phosphine-modified molecules.

These NHS-ester compounds react to form covalent bonds with primary amines (e.g., side chain of lysine residues or aminosilane-coated surfaces). The azide (N3) group reacts with phosphine-labeled molecules by a mechanism known as Staudinger chemistry, enabling efficient and specific conjugation of derivatized molecules in biological samples. In combination with different phosphine-activated compounds, these NHS-Azide reagents facilitate a variety of labeling and crosslinking experimental strategies for investigating protein interactions and cellular pathways.

Highlights:

• Soluble – reagents easily dissolve in water-miscible solvents for subsequent dilution in aqueous reaction mixtures with cell lysates and other biological samples

• Compatible – reaction chemistry occurs effectively in simple buffer conditions; requires no accessory reagents such as copper or reducing agents

• Specific – NHS esters are specific for covalent attachment to primary amines (side chain of lysine or N-terminus of polypeptides)

 

• Chemoselective – azide and phosphine groups do not react or interfere with components of biological samples but conjugate to one another with high efficiency

• Pegylated – choose the very short NHS-Azide or one of the two varieties whose spacer arms contain medium (4-unit) or long (12-unit) polyethylene glycol (PEG) groups for increased solubility and reach

Specifications

• Chemical Reactivity: Amine-Phosphine
• Cleavable: No
• Description: NHS-Azide
• Molecular Weight (g/mol): 198.14
• PEGylated: No
• Spacer Arm Length: 2.5 Å
• Content And Storage: Upon receipt store at -20°C protected from light and moisture.
• Cell Permeability: Not determined
• Shipping Condition: Ambient
• Product Line: Pierce™
• Labeling Method: Chemical Labeling
• Crosslinker Type: Heterobifunctional
• Reactive Moiety: Amine, Azide, NHS Ester
• Spacer: Short (<10 Å)
• Form: Powder
• Quantity: 10 mg
• Solubility: DMF, DMSO
• Format: Standard
• Water Soluble: No
• Product Type: Crosslinker

For Research Use Only. Not for use in diagnostic procedures.