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Thermo Scientific™ Pierce™ Ni-NTA Magnetic Agarose Beads
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Quantity:
4 mL
20 mL
Description
Product features:
Ni-NTA Magnetic Agarose Beads are used for small-scale affinity purification as well as high-throughput screening of recombinant His-tagged proteins. The polyhistidine tag is the most popular affinity tag and typically consists of six consecutive histidine residues (6xHis). Tagged proteins are overexpressed in a number of different systems, most commonly in bacteria, and purified from cell lysates such as those prepared using Thermo Scientific B-PER Bacterial Protein Extraction reagents. Purification of His-tagged proteins is achieved using an NTA chelate charged with nickel that coordinates with the histidine side chains. The NTA chelate contains four metal-binding sites that allow for low metal ion leaching and high binding capacity. The protocol for the Ni-NTA Magnetic Agarose Beads has been optimized to allow for high purity of the isolated His-tagged protein.
Ni-NTA Magnetic Agarose Beads are used for small-scale affinity purification as well as high-throughput screening of recombinant His-tagged proteins. The polyhistidine tag is the most popular affinity tag and typically consists of six consecutive histidine residues (6xHis). Tagged proteins are overexpressed in a number of different systems, most commonly in bacteria, and purified from cell lysates such as those prepared using Thermo Scientific B-PER Bacterial Protein Extraction reagents. Purification of His-tagged proteins is achieved using an NTA chelate charged with nickel that coordinates with the histidine side chains. The NTA chelate contains four metal-binding sites that allow for low metal ion leaching and high binding capacity. The protocol for the Ni-NTA Magnetic Agarose Beads has been optimized to allow for high purity of the isolated His-tagged protein.
- High capacity—sufficient for both routine and demanding purification procedures; binds >70 mg of 6xHis-tagged protein per mL of settled resin
- High-performance and reproducible beads—non-aggregating, magnetite (Fe3O4), superparamagnetic beads provide exceptional uniformity for both manual and automated HTS purification applications
- Low nonspecific binding— optimized purification protocol results in better tagged-protein purification
- Versatile—purify proteins using native or denaturing conditions
- Compatible—use with Thermo Scientific Cell Lysis reagents and a variety of buffer additives
- Protein purification and isolation
- High throughput protein purification using automated magnetic particle processor
Specifications
Specifications
| Content And Storage | 1 mL settled resin. Supplied as 4 mL of 25% v/v suspension in 20% ethanol. Store at 2–8°C. |
| Shipping Condition | Wet Ice |
| Form | Liquid Suspension |
| Product Line | Pierce™ |
| Quantity | 4 mL |
| Protein Form | Recombinant |
| Type | Magnetic Agarose Bead |
| Stationary Phase | Metal Chelate, Divalent Nickel |
| Format | Bottle |
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