Thermo Scientific™ Peptide Retention Standard

A mixture of five specific deca-peptides (acetylated with c-terminal amides) to run reference elution profiles for reverse-phase HPLC chromatography.

Thermo Scientific™ Pierce Peptide Retention Standard is a mixture of five c-terminal amide decapeptides that provide reference elution profiles in reverse-phase HPLC.

Retention times are predicted by totaling the values that represent the contribution in minutes of each amino acid residue and the peptide terminal groups. Retention time is dependent upon the molecular weight of the peptide. The effect on retention is relatively unimportant with a small peptide, but it increases with the size of the molecule. The accuracy of predicting peptide retention time significantly decreases beyond 20 residues. To ensure accuracy, a peptide standard is used to correct for instrument variation, column aging, n-alkyl chain length variation and ligand density.

Highlights:

• Save time in peptide purification
• Simplify identification of specific peptides in a complex mixture
• Increase the efficiency of predicting peptide elution profiles Determine the relative order of peptide elution of a complex mixture Predict the HPLC retention time for peptides of known amino acid composition on reverse phase HPLC columns Monitor column performance: efficiency, selectivity and resolution during column aging Compare reverse-phase columns from different manufacturers Evaluate reverse-phase supports of varying n-alkyl chain lengths and ligand densities

For Use With (Equipment)

HPLC

Product Type

Peptide Retention Standard

Content And Storage

Upon receipt store at -20°C. Product is shipped at ambient temperature.

Starting Material

Peptides

Detection Method

HPLC

Workflow Step

Standardization

Quantity

1 Vial

Form

Solid

For Research Use Only. Not for use in diagnostic procedures.