Thermo Scientific™ Pierce™ Peroxide Assay Reagent C

Detect and measure hydrogen peroxide levels (H2O2) in biological samples using an iron (Fe) and xylenol orange (XO) reagent for microplates or tubes.

Detect and measure hydrogen peroxide levels in biological samples using an iron and xylenol orange reagent with Thermo Scientific™ Pierce Quantitative Peroxide Assay Kits.

These aqueous and lipid-compatible peroxide assay kits detect peroxides based on oxidation of ferrous to ferric ion in the presence of xylenol orange. One assay kit has an aqueous-compatible formulation that includes sorbitol, which provides sensitivity enhancement. The other assay kit has a formulation that may be used with lipid-containing samples without first extracting the lipids; however, because this kit does not contain sorbitol, the assay is less sensitive. Most proteins do not interfere with these assays, although some metal chelators may require use of a blank (i.e., control) to mitigate these effects.

In these assays, hydroperoxides convert the Fe2+ to Fe3+ at acidic pH. With the aqueous-compatible formulation, peroxide first reacts with sorbitol, converting it to a peroxyl radical, which in turn initiates Fe2+ oxidation to Fe3+. In the lipid compatible formulation, the peroxide converts the Fe2+ to Fe3+ directly. In a sulfuric acid solution, the Fe3+ complexes with the xylenol orange (XO) dye to yield a purple product having an absorbance maximum at 560nm. Peroxide levels in test samples can be determined by calculation from the know extinction coefficient of the XO-Fe complex or by reference to a standard curve prepared with hydrogen peroxide solution.

Highlights:

• Peroxides – detects and measures hydrogen peroxide (H2O2) levels in biological and other liquids samples

• Convenient – no proprietary reagents involved but the ready-to-use kits remove the variability, difficulty and added expense of formulating the reagents yourself

• Versatile – quantitation by calculation from the extinction coefficient or by reference to a standard curve enables the method to be easily adapted to a variety of sample types and testing purposes

• Two formulations – choose the aqueous-compatible kit for maximum sensitivity with non-lipid samples; choose the lipid-compatible kit for best results with lipid-containing samples

Recommended for:

General hydrogen peroxide (H²O²) measurement in protein samples; Quantitation of peroxides in detergents; Assessment of the effects of peroxides on cellular activity; Determination of protein glycation; Measurement of peroxide accumulation in lipids

Content And Storage

Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 2 to 8°C.

Description

Pierce Peroxide Assay Reagent C

Assay

Peroxide Assay Reagent C

Detection Method

Colorimetric

Product Line

Pierce™

Product Type

Quantitative Peroxide Assay

Specificity

Not Target-Specific

For Use With (Application)

Solution-based Detection, Absorbance

For Use With (Equipment)

Spectrophotometer, Microplate Reader

For Research Use Only. Not for use in diagnostic procedures.