Thermo Scientific™ EZ-Link™ Plus Activated Peroxidase Kit

Periodate-aldehyde-activated horseradish peroxidase (HRP) enzyme and kit to label primary antibodies to obtain high-activity antibody-HRP conjugates.

Thermo Scientific™ EZ-Link™ Plus Activated Peroxidase Kit contains horseradish peroxidase enzyme that has been aldehyde-activated with periodate to enable high-efficiency labeling and preparation of protein or antibody conjugates.

Other amine-reactive chemistries, like glutaraldehyde, have a tendency to polymerize and produce lower amounts of viable conjugate. Sugar residues present on HRP have been oxidized with periodate to produce aldehydes that react with primary amines. Plus Activated Peroxidase is mixed with the protein to be coupled at a pH compatible with your protein or antibody. After incubating the reaction for one to two hours, the conjugate linkage is reduced and the activated HRP quenched with ethanolamine. The conjugate is purified by desalting or by dialysis. If you are preparing an antibody-HRP conjugate, use the Thermo Scientific Pierce Conjugate Purification Kit (Part No. 44920) to separate unreacted enzyme from the conjugate.

Highlights:

• Reacts with readily accessible primary amines to form a covalent secondary amine bond
• Can be stored for at least 12 months at -20°C
• Consistent conjugation yields reliable conjugates
• Enzyme activity is 120-200 units/mg

Quantity

1-plate kit

Content And Storage

Sufficient For: 5 enzyme-labeling reactions, each with up to 1 mg of antibody (IgG)
• Plus Activated Peroxidase, 5 x 1 mg
• Sodium cyanoborohydride solution, 1 x 0.5 mL
• Quenching Buffer, 25 mL
• PBS Pack (makes 500 mL), 1 pack
• Carbonate-Bicarbonate Buffer Pack (makes 500 mL), 1 pack

Store at -20°C.

Product Line

EZ-Link™

Sufficient For

5 Enzyme Labeling Reactions

Labeling Target

Antibodies (General), Proteins (General)

Label or Dye

HRP (Horseradish Peroxidase)

Labeling Scale

0.5-1 mg

For Research Use Only. Not for use in diagnostic procedures.