Thermo Scientific™ Pierce™ Polyacrylamide Spin Desalting Columns, 7K MWCO, 0.7 mL

Separate proteins and other macromolecules from low-molecular weight buffer salts and reagents with Thermo Scientific Pierce 7K MWCO (molecular-weight cutoff) Polyacrylamide Spin Desalting Columns, which are porous polyacrylamide-based gel-filtration columns for biological sample preparation.

Desalting and buffer exchange are two of the most widely used applications in resin filtration chromatography. Thermo Scientific Pierce Polyacrylamide Spin Desalting Columns, 7K MWCO (molecular-weight cutoff), are ready-to-use, disposable, gel-filtration columns for the separation of proteins and other macromolecules from low-molecular-weight buffer salts and reagents.

Pierce Polyacrylamide Spin Desalting Columns, 7K MWCO, are pre-packed with porous polyacrylamide beads and enable buffer-exchange and desalting of proteins and other macromolecular samples based on a molecular-weight cutoff (MWCO) of 7000 Da. Unlike other size-exclusion media, polyacrylamide resin is not subject to enzymatic degradation and will not serve as a nutrient for microbial growth. The resin is very hydrophilic, thereby minimizing undesirable binding interactions between the resin and sample molecules. Contamination with low molecular weight sugars (which may occur with crosslinked dextran resin) is not a concern when using polyacrylamide resin. Oxidizing agents can be removed without destroying the support. This resin is susceptible to hydrolysis of amide groups under extreme pH conditions, so an operating pH of 2–10 is recommended at room temperature. The polyacrylamide resin can also be autoclaved at pH 5.5–6.5 for 30 minutes at 120°C.

The columns use a resin with beads that have a wet diameter of 45 to 90 μM and are ideal for separating peptides and small macromolecules (>7 kDa) from buffer salts and other compounds (less than 1K MWCO).

Features of polyacrylamide resin include:
• Stable in water, salt solutions, organic solvents, and alkaline or acidic conditions
• Excellent flow properties
• Heat stable

Applications
• Removing salts from protein solutions
• Removing phenol from nucleic acid preparations
• Separating excess crosslinker from conjugate preparations
• Removing excess derivatizing agents from modified proteins
• Removing unreacted dye from fluorescent antibodies
• Removing free radiolabel from labeled proteins
• Buffer exchange

Description

Pierce™ Polyacrylamide Spin Desalting Columns, 7K MWCO, 0.7 mL

Product Type

Spin Desalting Column

Content And Storage

Upon receipt store at 4°C.

Format

Spin Column

Purification Target

Buffer Exchange, Protein

Column Type

Size-exclusion, Polyacrylamide Resin

MWCO

7.0 kDa

Product Line

Pierce™

Quantity

25 Columns

Sample Volume (Metric)

30 to 120 μL